LOCUS       sequence-220555-        9600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to rab20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actc1b-mKate2-rab20
FEATURES             Location/Qualifiers
     source          1..9600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1424..1444)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(1452..1468)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1484..1618
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1508..1527)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1562..1581
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(2057..2244,2245..2256)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2290..2306)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2290..2306)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2343..2363)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2343..2361)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2382..2398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2395..2417)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2406..2422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2430..2436,2437..2454,2455..2460))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2475..2496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2613..2630)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2784..3372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2864..2883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3543..4334,4335..4403))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4166..4185
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4404..4508)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4671..4692)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5041..5052,5053..5215)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5588..5604
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5624..5644
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggtgaacgtt ggaaagactt ctctcctcca
      781 cagatacacg gagaggaagt ttaaggacac gataagcacc gtcggagggg cgtttttcct
      841 caaacagtgg ggtccctaca acatttccat atgggatact gcaggtcgag agcagtttca
      901 cggtctgggc tccatgtact gccgtggcgc agctgctgtc atcctaacat atgatgtcac
      961 caactggcag agcctggcgg agctggagaa ccgcttcctg tccttgactg acactgccaa
     1021 cgcagactgc atcttcgccc ttgtggggaa caaagcggac ctgaccgacc cgttcgctca
     1081 agagcctgac agcgagagtg ggagcgaccc gcagcctgta gcctcctgcc cgactcctcc
     1141 tgccagccct gtccagcaca aacaggtgag cagagaggac gctgtggctc tgtacgcccg
     1201 cgttctccga tacaaaggcc tggacgagaa gacctgcctg cccgccgaga ggatgtgctt
     1261 cgagaccagc gctaaaacag gttacaacgt ggacgctttg ttcgagacct tatttgattt
     1321 agtactgcca tccattctga agaagaggag cgagggtgaa gccactgtag atctggagac
     1381 gccactagag gtgagaagga ctaaagctgg ctgctgtgca tagcaacttt attatacata
     1441 gttgataatt cactggccgt cgttttacgg taccatcgat gatgatccag acatgataag
     1501 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     1561 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     1621 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     1681 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag atcagatctg
     1741 cgaagatacg gccacgggtg ctcttgatcc tgtggctgat tttggactgt gctgctcgca
     1801 gctgctgatg aatcacatac ttcctccatt ttcttccact gattgactgt tataatttcc
     1861 ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga cgtcacttcc
     1921 aaaggaccaa tgaacatgtc tgaccaattt catataatgt gaaaacgatt ttcataggca
     1981 gaataaataa catttaaatt aaactgggca tcagcgcaat tcaattggtt tggtaatagc
     2041 aagggaaaat agaatgaagt gatctccaaa aaataagtac tttttgactg taaataaaat
     2101 tgtaaggagt aaaaagtact tttttttcta aaaaaatgta attaagtaaa agtaaaagta
     2161 ttgattttta attgtactca agtaaagtaa aaatccccaa aaataatact taagtacagt
     2221 aatcaagtaa aattactcaa gtactttaca cctctggttc ttgaccccct accttcagca
     2281 agcccagcag atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
     2341 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     2401 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2461 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2521 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2581 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2641 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2701 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2761 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2941 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3181 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3361 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3421 aactcacgtt aaggcatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3481 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3541 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3601 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3661 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3721 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3781 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3841 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3901 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3961 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4021 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4081 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4141 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4201 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4261 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4321 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4381 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4441 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4501 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     4561 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     4621 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     4681 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     4741 agggcgatgg cccacgctgg ctaagaactc atcagcctcc ccggtccatc tacccacgta
     4801 ccaatgcacc aattggccac aatgacggct actacatggt gccattcctt cctctttata
     4861 ggaatggaga ctacctcctg tccaacaatg ctcttggata cgagtacgcc tacctgttgg
     4921 acccaggtca ttgcacaaca ccagaaatgc cctctgatct gcaaaagacg tgaatatctg
     4981 ttcagacacc catatccact ctgttccaca caggtcagag gtttgtccag gagttcttga
     5041 cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa
     5101 attttactca attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa
     5161 agtactccat taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa
     5221 actagagatt cttgtttaag cttttaatct caaaaaacat taaatgaaat gcatacaagg
     5281 ttttatcctg ctttagaact gtttgtattt aattatcaaa ctataagaca gacaatctaa
     5341 tgccagtaca cgctactcaa agttgtaaaa cctcagattt aacttcagta gaagctgatt
     5401 ctcaaaattg ttagtgtcaa gcctagctct tttggggctg aaaagcaatc ctgcagtgct
     5461 gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta ttagtcttga tagagaggct
     5521 gcaaatagca ggaaacgtga gcagagactc cctggtgtct gaaacacagg ccagatgggc
     5581 cctcgagcag gaaacagcta tgaccatgat tacgccaagc tatcaacttt gtatagaaaa
     5641 gttggcacgc gcagattccg tgtgggcctg gtaatatctt accattttaa gctattttaa
     5701 gattagggaa aaaaattaaa taaaactact ttaaaattac tttccttaaa aagcaacata
     5761 tttatttagt ttttgtttaa ctggctcaag attgtaatat tttactttta catgtttttt
     5821 taagtaccta aatgtgttca aaagtaaagt atgttattgt tttttttttt taatgttagc
     5881 taagctttaa attgtaatat attactttta aaagtgactt tttaaaaatg taaataaaaa
     5941 agtaactata aatgtgtaat gcatgttatt tctgtgggtt tttaattttc atctcaactg
     6001 ggcttacaat tttaacataa taaaaatcaa atcaaatcaa atcaggccct tatttgtcac
     6061 atacactctt gtatactgaa ataaactaaa tgttgagaaa aatttaaata caccaaaata
     6121 acgcatacct tttttgtaat tacctaagta aaacatttag ttcctttttt aagtgactca
     6181 agattgtaat ctattaattg taaaagataa aactagtgat caatctgacg tgcttctatg
     6241 cagcctgtga gattaattgt gatgtttaat tattaaacaa tttaaatgga tcaaacattt
     6301 cacatgtgct tattgtgtcc aacaaaacca gcagaagcac ttaaaaatat tgcatgacaa
     6361 catgagtttt taatgtaaat tatatatact tcctaaacga agttaatcat atttctaaaa
     6421 taataatgtc attgaaatat ttaaataaat gataagcttt agttgattca acataaaatt
     6481 gaaagcaata aggacttttt ttttttacag cggtgaagca aaagcatgta ataatcatat
     6541 aatataataa ataaataaat aaataatggc tcttggtgca aatatgaaca ctttaaataa
     6601 atatagaata aatatgatca ataagtcata acgatgtgtt ttatgttgat tataatttaa
     6661 acatgtttct aacgcaagtt ttaaaaactg ttttaaagta ttattttaag ttataagatc
     6721 aatttgactc aacattttgt agtgaaaata tgttgtgtaa aattaatata atatacacta
     6781 taaaaatgca gggttccaca caatacatta atgttgtccc aacacaaatc gattaagtta
     6841 acaaatatat gtggattgaa cataaaaaat taagttgtcc caatgaaatc tcaagaattg
     6901 tgttgtttca gctcatttta aataagtagt tttaacaagt aaaaaataac atatttttga
     6961 gtgtgtatat atatatatta caaaatataa taatataaaa tgtaaaagat ataaaccatg
     7021 ctacatatat ttaagagcta ttttgcacat tacttttttg caagccattt tagtgttttt
     7081 tttacaaata tatattagct aaatatacaa tatcttaaat atacaattaa tcagacatgc
     7141 aattaatgca tttaacctac ataaaaatac atgatcaaca tatgttttat gctgattaca
     7201 actaaatcaa acgtaatgaa attaatttat aaaagcgatt taaatatgtc aatattttaa
     7261 gttaaaatca cttgaaacct actataaatg aacttaaatt aaaaaaatct cctttaattc
     7321 caatgcaaca gtgttgtttt ttcagctgag gacccccggt gcagatggtt ggcccccggc
     7381 ccgctattct aaggaagcct ttcttaattt tagggcggcg ctgcatgcgc tcagatcaga
     7441 ttcacgggtg gagagggtta ttttctcttt tttatttttt cttgcctgct catatttggg
     7501 cctgtgtgaa ccccaggctc cgcctcggcc cccaggagcc gctcttattt aaaggggatc
     7561 ctcgccttca gtcttcgtcc cagtggaagc atcagtcttg tgataacaac ttggtgagtc
     7621 ctacagacca gcgctcgact ctaggcttta aatgcattga tttctctgga ttagctaccg
     7681 ggagagtttt aactttgctt attagtcttg tgctcgaact gctaggttta aagagtgttt
     7741 tctctaatgc atgcatgaag cgacagctga aaaccagaaa tagagcgttt gtcctcctgt
     7801 taaactctag cctttctttg gcatttgtca gtccgagtct tcagctgccg gcaggagatt
     7861 gggtttcagt aacacttgct gagcacaaac tacacgtgct ttcttcagat ttaattcatt
     7921 attaatcatt ctagatttaa atgatgttct tactcattgc tgattatttt agccttgcgg
     7981 agcagattaa atgagcacgt gtttcattaa gattgattta aatataatta atatttcacc
     8041 actgtagttt taaaattgcc ttctaagtca tggttgattt ttaataggta tcggctctaa
     8101 ttagattact attttggttt ttaaatgtta tttctatttc atttgtaggc tcgtttttaa
     8161 tgtgatttta ttagattcta gttttaaaat catctcttag ccttaatgag cagactgaat
     8221 gaacatgttt cattaagatt aacttcatta taatgaatta tttaatcatt ctagtgggtt
     8281 ataaatcagc tctatttaaa ttaattgtcg tgtttatttc tatttaattt ttgattattt
     8341 tttcagggtc gtttttatgt gattttttcc cgtttctaat tttaaactaa ttgtttgatt
     8401 tttatatgat tcgactcttt ttaaatgaat tgttttgctt tttttattga gcatatatat
     8461 attatacaat ttcaatcaac aattgttgtg ctttttaaat gttgtttcta tttcatttct
     8521 agggtcgatt ttattcgatt ctagttttaa aatcgtgatt tttaacttct aaccttactg
     8581 agcagactga atgaacatgt ttcatcaaga ttaacttcat tatagttaat tattgaatcg
     8641 tgctagtggt ttataaatca gctctattta aattaatcat catcattatt tctatttaat
     8701 ttttattatt tctttaggat tatttttatg tgattttttt cacgttctag ttttaaactc
     8761 aatgctcatt actgctcacc cttgctgagc agtctttcgt taagattaaa ttaattaata
     8821 accattctag ttttaaaaca gtgtcttaag gcatagctga tattttacaa gtttactatt
     8881 taatatatct cctttctttt tttagggtcg atttcatctg atttgattaa tttttattat
     8941 cttcactaag ctttttgttc taggttttat tctcagtatt attactgtta tatttgaact
     9001 gaatttctcc tcatagttta ttcattttat ttatttattt tttgtttatt cgctattttg
     9061 tattttagtt tttaattaga aattttatta gaatcgtttt gaactagttt ttatgcactc
     9121 taacattaat attaaatcat aactattatt tttattataa tttatttgtg tttatctttt
     9181 tgtgtagctt tcatgtgttt ttattttagt tttattatgt ttgcattgaa ttttttggac
     9241 tagtttatat aacgatatac agaaaagcca tgacaacaaa tgtttttata atacctatta
     9301 agtataagct aatcagattt ccactattct gttcaaagtt aacattatta gccattctga
     9361 tggatcattt gacatttgtc atatgtcttt atgaacactg ctctttcaga tgccactttt
     9421 aaagctgttt tttacagtaa atgaacgttt attattatga cattatttcc taaggcctct
     9481 agagtacagc aggatgtggc gtgtgcatta ccgtgtaatt atgctgactg taaatgtgca
     9541 tttatagact gtcagcatgt ctgactgtgc gctgttgtga ttggtcctgc acagaccaag
//
