LOCUS       sequence-220558-        9860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized enAsBE1.3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UG
            I-NLS(SV40)(enAsBE1.3) (RTW1296)
FEATURES             Location/Qualifiers
     source          1..9860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1771..1780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1777..2466
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MGSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINW
                     GGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEF
                     LSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPS
                     NEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPH
                     ILWATGLK"
     CDS             2563..6480
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     CDS             6511..6759
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6772..6792
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6882..6901)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6947..7002
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7001..7020)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7363..7379)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7363..7379)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7376..7398)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7387..7403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7411..7417,7418..7435,7436..7441))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7456..7477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7536..7731
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7582..7717
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7737..7871
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7774..7793)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7828..7847
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7939..7956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8110..8698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8190..8209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8869..9660,9661..9729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9492..9511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9730..9834)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggsgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tctgcagtcg
     1741 acggtaccgc gggcccggga tccaccggtc gccaccatgg gcagctcaga gactggccca
     1801 gtggctgtgg accccacatt gagacggcgg atcgagcccc atgagtttga ggtattcttc
     1861 gatccgagag agctccgcaa ggagacctgc ctgctttacg aaattaattg ggggggccgg
     1921 cactccattt ggcgacatac atcacagaac actaacaagc acgtcgaagt caacttcatc
     1981 gagaagttca cgacagaaag atatttctgt ccgaacacaa ggtgcagcat tacctggttt
     2041 ctcagctgga gcccatgcgg cgaatgtagt agggccatca ctgaattcct gtcaaggtat
     2101 ccccacgtca ctctgtttat ttacatcgca aggctgtacc accacgctga cccccgcaat
     2161 cgacaaggcc tgcgggattt gatctcttca ggtgtgacta tccaaattat gactgagcag
     2221 gagtcaggat actgctggag aaactttgtg aattatagcc cgagtaatga agcccactgg
     2281 cctaggtatc cccatctgtg ggtacgactg tacgttcttg aactgtactg catcatactg
     2341 ggcctgcctc cttgtctcaa cattctgaga aggaagcagc cacagctgac attctttacc
     2401 atcgctcttc agtcttgtca ttaccagcga ctgcccccac acattctctg ggccaccggg
     2461 ttgaaatctg gtggttcttc tggtggttct agcggcagcg agactcccgg gacctcagag
     2521 tccgccacac ccgaaagttc cggagggagt agcggcgggt ctacacagtt cgagggcttt
     2581 accaacctgt atcaggtgag caagacactg cggtttgagc tgatcccaca gggcaagacc
     2641 ctgaagcaca tccaggagca gggcttcatc gaggaggaca aggcccgcaa tgatcactac
     2701 aaggagctga agcccatcat cgatcggatc tacaagacct atgccgacca gtgcctgcag
     2761 ctggtgcagc tggattggga gaacctgagc gccgccatcg actcctatag aaaggagaaa
     2821 accgaggaga caaggaacgc cctgatcgag gagcaggcca catatcgcaa tgccatccac
     2881 gactacttca tcggccggac agacaacctg accgatgcca tcaataagag acacgccgag
     2941 atctacaagg gcctgttcaa ggccgagctg tttaatggca aggtgctgaa gcagctgggc
     3001 accgtgacca caaccgagca cgagaacgcc ctgctgcgga gcttcgacaa gtttacaacc
     3061 tacttctccg gcttttatag aaacaggaag aacgtgttca gcgccgagga tatcagcaca
     3121 gccatcccac accgcatcgt gcaggacaac ttccccaagt ttaaggagaa ttgtcacatc
     3181 ttcacacgcc tgatcaccgc cgtgcccagc ctgcgggagc actttgagaa cgtgaagaag
     3241 gccatcggca tcttcgtgag cacctccatc gaggaggtgt tttccttccc tttttataac
     3301 cagctgctga cacagaccca gatcgacctg tataaccagc tgctgggagg aatctctcgg
     3361 gaggcaggca ccgagaagat caagggcctg aacgaggtgc tgaatctggc catccagaag
     3421 aatgatgaga cagcccacat catcgcctcc ctgccacaca gattcatccc cctgtttaag
     3481 cagatcctgt ccgataggaa caccctgtct ttcatcctgg aggagtttaa gagcgacgag
     3541 gaagtgatcc agtccttctg caagtacaag acactgctga gaaacgagaa cgtgctggag
     3601 acagccgagg ccctgtttaa cgagctgaac agcatcgacc tgacacacat cttcatcagc
     3661 cacaagaagc tggagacaat cagcagcgcc ctgtgcgacc actgggatac actgaggaat
     3721 gccctgtatg agcggagaat ctccgagctg acaggcaaga tcaccaagtc tgccaaggag
     3781 aaggtgcagc gcagcctgaa gcacgaggat atcaacctgc aggagatcat ctctgccgca
     3841 ggcaaggagc tgagcgaggc cttcaagcag aaaaccagcg agatcctgtc ccacgcacac
     3901 gccgccctgg atcagccact gcctacaacc ctgaagaagc aggaggagaa ggagatcctg
     3961 aagtctcagc tggacagcct gctgggcctg taccacctgc tggactggtt tgccgtggat
     4021 gagtccaacg aggtggaccc cgagttctct gcccggctga ccggcatcaa gctggagatg
     4081 gagccttctc tgagcttcta caacaaggcc agaaattatg ccaccaagaa gccctactcc
     4141 gtggagaagt tcaagctgaa ctttcagatg cctacactgg ccagaggctg ggacgtgaat
     4201 agagagaaga acaatggcgc catcctgttt gtgaagaacg gcctgtacta tctgggcatc
     4261 atgccaaagc agaagggcag gtataaggcc ctgagcttcg agcccacaga gaaaaccagc
     4321 gagggctttg ataagatgta ctatgactac ttccctgatg ccgccaagat gatcccaaag
     4381 tgcagcaccc agctgaaggc cgtgacagcc cactttcaga cccacacaac ccccatcctg
     4441 ctgtccaaca atttcatcga gcctctggag atcacaaagg agatctacga cctgaacaat
     4501 cctgagaagg agccaaagaa gtttcagaca gcctacgcca agaaaaccgg cgaccagaag
     4561 ggctacagag aggccctgtg caagtggatc gacttcacaa gggattttct gtccaagtat
     4621 accaagacaa cctctatcga tctgtctagc ctgcggccat cctctcagta taaggacctg
     4681 ggcgagtact atgccgagct gaatcccctg ctgtaccaca tcagcttcca gagaatcgcc
     4741 gagaaggaga tcatggatgc cgtggagaca ggcaagctgt acctgttcca gatctataac
     4801 aaggactttg ccaagggcca ccacggcaag cctaatctgc acacactgta ttggaccggc
     4861 ctgttttctc cagagaacct ggccaagaca agcatcaagc tgaatggcca ggccgagctg
     4921 ttctaccgcc ctaagtccag gatgaagagg atggcacacc ggctgggaga gaagatgctg
     4981 aacaagaagc tgaaggatca gaaaacccca atccccgaca ccctgtacca ggagctgtac
     5041 gactatgtga atcacagact gtcccacgac ctgtctgatg aggccagggc cctgctgccc
     5101 aacgtgatca ccaaggaggt gtctcacgag atcatcaagg ataggcgctt taccagcgac
     5161 aagttctttt tccacgtgcc tatcacactg aactatcagg ccgccaattc cccatctaag
     5221 ttcaaccaga gggtgaatgc ctacctgaag gagcaccccg agacacctat catcggcatc
     5281 gcccggggcg agagaaacct gatctatatc acagtgatcg actccaccgg caagatcctg
     5341 gagcagcgga gcctgaacac catccagcag tttgattacc agaagaagct ggacaacagg
     5401 gagaaggaga gggtggcagc aaggcaggcc tggtctgtgg tgggcacaat caaggatctg
     5461 aagcagggct atctgagcca ggtcatccac gagatcgtgg acctgatgat ccactaccag
     5521 gccgtggtgg tgctggagaa cctgaatttc ggctttaaga gcaagaggac cggcatcgcc
     5581 gagaaggccg tgtaccagca gttcgagaag atgctgatcg ataagctgaa ttgcctggtg
     5641 ctgaaggact atccagcaga gaaagtggga ggcgtgctga acccatacca gctgacagac
     5701 cagttcacct cctttgccaa gatgggcacc cagtctggct tcctgtttta cgtgcctgcc
     5761 ccatatacat ctaagatcga tcccctgacc ggcttcgtgg accccttcgt gtggaaaacc
     5821 atcaagaatc acgagagccg caagcacttc ctggagggct tcgactttct gcactacgac
     5881 gtgaaaaccg gcgacttcat cctgcacttt aagatgaaca gaaatctgtc cttccagagg
     5941 ggcctgcccg gctttatgcc tgcatgggat atcgtgttcg agaagaacga gacacagttt
     6001 gacgccaagg gcaccccttt catcgccggc aagagaatcg tgccagtgat cgagaatcac
     6061 agattcaccg gcagataccg ggacctgtat cctgccaacg agctgatcgc cctgctggag
     6121 gagaagggca tcgtgttcag ggatggctcc aacatcctgc caaagctgct ggagaatgac
     6181 gattctcacg ccatcgacac gatggtggcc ctgatccgca gcgtgctgca gatgcggaac
     6241 tccaatgccg ccacaggcga ggactatatc aacagccccg tgcgcgatct gaatggcgtg
     6301 tgcttcgact cccggtttca gaacccagag tggccaatgg acgccgatgc caatggcgcc
     6361 taccacatcg ccctgaaggg ccagctgctg ctgaatcacc tgaaggagag caaggatctg
     6421 aagctgcaga acggcatctc caatcaggac tggctggcct acatccagga gctgcgcaac
     6481 tctggtggtt ctggaggatc tggtggttct actaatctgt cagatattat tgaaaaggag
     6541 accggtaagc aactggttat ccaggaatcc atcctcatgc tcccagagga ggtggaagaa
     6601 gtcattggga acaagccgga aagcgatata ctcgtgcaca ccgcctacga cgagagcacc
     6661 gacgagaatg tcatgcttct gactagcgac gcccctgaat acaagccttg ggctctggtc
     6721 atacaggata gcaacggtga gaacaagatt aagatgctct ctggtggttc tcccaagaag
     6781 aagaggaaag tctaaagcgg ccgcactcct caggtgcagg ctgcctatca gaaggtggtg
     6841 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     6901 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     6961 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     7021 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     7081 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     7141 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     7201 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     7261 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     7321 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     7381 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7441 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7501 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
     7561 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7621 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7681 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
     7741 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7801 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7861 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7921 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7981 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8041 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8101 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8161 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8221 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8281 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8341 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8401 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8461 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8521 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8581 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8641 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8701 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8761 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8821 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8881 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8941 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9001 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9061 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9121 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9181 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9241 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9301 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9361 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9421 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9481 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9541 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9601 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9661 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9721 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9781 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9841 tccccgaaaa gtgccacctg
//
