LOCUS       sequence-220564-       10498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized 
            AsCas12a-VPR(1.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 10498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)
FEATURES             Location/Qualifiers
     source          1..10498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1777..5697
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     CDS             5698..5745
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5752..5832
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5863..6012
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6037..6057
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6487..6843
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             6862..7431
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     primer_bind     complement(7520..7539)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7585..7640
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7639..7658)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8001..8017)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8001..8017)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8014..8036)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8025..8041
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8049..8055,8056..8073,8074..8079))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8094..8115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8174..8369
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8220..8355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8375..8509
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8412..8431)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8466..8485
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8577..8594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8748..9336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8828..8847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9507..10298,10299..10367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRLA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10130..10149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10368..10472)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tctgcagtcg
     1741 acggtaccgc gggcccggga tccaccggtc gccaccatga cacagttcga gggctttacc
     1801 aacctgtatc aggtgagcaa gacactgcgg tttgagctga tcccacaggg caagaccctg
     1861 aagcacatcc aggagcaggg cttcatcgag gaggacaagg cccgcaatga tcactacaag
     1921 gagctgaagc ccatcatcga tcggatctac aagacctatg ccgaccagtg cctgcagctg
     1981 gtgcagctgg attgggagaa cctgagcgcc gccatcgact cctatagaaa ggagaaaacc
     2041 gaggagacaa ggaacgccct gatcgaggag caggccacat atcgcaatgc catccacgac
     2101 tacttcatcg gccggacaga caacctgacc gatgccatca ataagagaca cgccgagatc
     2161 tacaagggcc tgttcaaggc cgagctgttt aatggcaagg tgctgaagca gctgggcacc
     2221 gtgaccacaa ccgagcacga gaacgccctg ctgcggagct tcgacaagtt tacaacctac
     2281 ttctccggct tttatgagaa caggaagaac gtgttcagcg ccgaggatat cagcacagcc
     2341 atcccacacc gcatcgtgca ggacaacttc cccaagttta aggagaattg tcacatcttc
     2401 acacgcctga tcaccgccgt gcccagcctg cgggagcact ttgagaacgt gaagaaggcc
     2461 atcggcatct tcgtgagcac ctccatcgag gaggtgtttt ccttcccttt ttataaccag
     2521 ctgctgacac agacccagat cgacctgtat aaccagctgc tgggaggaat ctctcgggag
     2581 gcaggcaccg agaagatcaa gggcctgaac gaggtgctga atctggccat ccagaagaat
     2641 gatgagacag cccacatcat cgcctccctg ccacacagat tcatccccct gtttaagcag
     2701 atcctgtccg ataggaacac cctgtctttc atcctggagg agtttaagag cgacgaggaa
     2761 gtgatccagt ccttctgcaa gtacaagaca ctgctgagaa acgagaacgt gctggagaca
     2821 gccgaggccc tgtttaacga gctgaacagc atcgacctga cacacatctt catcagccac
     2881 aagaagctgg agacaatcag cagcgccctg tgcgaccact gggatacact gaggaatgcc
     2941 ctgtatgagc ggagaatctc cgagctgaca ggcaagatca ccaagtctgc caaggagaag
     3001 gtgcagcgca gcctgaagca cgaggatatc aacctgcagg agatcatctc tgccgcaggc
     3061 aaggagctga gcgaggcctt caagcagaaa accagcgaga tcctgtccca cgcacacgcc
     3121 gccctggatc agccactgcc tacaaccctg aagaagcagg aggagaagga gatcctgaag
     3181 tctcagctgg acagcctgct gggcctgtac cacctgctgg actggtttgc cgtggatgag
     3241 tccaacgagg tggaccccga gttctctgcc cggctgaccg gcatcaagct ggagatggag
     3301 ccttctctga gcttctacaa caaggccaga aattatgcca ccaagaagcc ctactccgtg
     3361 gagaagttca agctgaactt tcagatgcct acactggcct ctggctggga cgtgaataag
     3421 gagaagaaca atggcgccat cctgtttgtg aagaacggcc tgtactatct gggcatcatg
     3481 ccaaagcaga agggcaggta taaggccctg agcttcgagc ccacagagaa aaccagcgag
     3541 ggctttgata agatgtacta tgactacttc cctgatgccg ccaagatgat cccaaagtgc
     3601 agcacccagc tgaaggccgt gacagcccac tttcagaccc acacaacccc catcctgctg
     3661 tccaacaatt tcatcgagcc tctggagatc acaaaggaga tctacgacct gaacaatcct
     3721 gagaaggagc caaagaagtt tcagacagcc tacgccaaga aaaccggcga ccagaagggc
     3781 tacagagagg ccctgtgcaa gtggatcgac ttcacaaggg attttctgtc caagtatacc
     3841 aagacaacct ctatcgatct gtctagcctg cggccatcct ctcagtataa ggacctgggc
     3901 gagtactatg ccgagctgaa tcccctgctg taccacatca gcttccagag aatcgccgag
     3961 aaggagatca tggatgccgt ggagacaggc aagctgtacc tgttccagat ctataacaag
     4021 gactttgcca agggccacca cggcaagcct aatctgcaca cactgtattg gaccggcctg
     4081 ttttctccag agaacctggc caagacaagc atcaagctga atggccaggc cgagctgttc
     4141 taccgcccta agtccaggat gaagaggatg gcacaccggc tgggagagaa gatgctgaac
     4201 aagaagctga aggatcagaa aaccccaatc cccgacaccc tgtaccagga gctgtacgac
     4261 tatgtgaatc acagactgtc ccacgacctg tctgatgagg ccagggccct gctgcccaac
     4321 gtgatcacca aggaggtgtc tcacgagatc atcaaggata ggcgctttac cagcgacaag
     4381 ttctttttcc acgtgcctat cacactgaac tatcaggccg ccaattcccc atctaagttc
     4441 aaccagaggg tgaatgccta cctgaaggag caccccgaga cacctatcat cggcatcgcc
     4501 cggggcgaga gaaacctgat ctatatcaca gtgatcgact ccaccggcaa gatcctggag
     4561 cagcggagcc tgaacaccat ccagcagttt gattaccaga agaagctgga caacagggag
     4621 aaggagaggg tggcagcaag gcaggcctgg tctgtggtgg gcacaatcaa ggatctgaag
     4681 cagggctatc tgagccaggt catccacgag atcgtggacc tgatgatcca ctaccaggcc
     4741 gtggtggtgc tggagaacct gaatttcggc tttaagagca agaggaccgg catcgccgag
     4801 aaggccgtgt accagcagtt cgagaagatg ctgatcgata agctgaattg cctggtgctg
     4861 aaggactatc cagcagagaa agtgggaggc gtgctgaacc cataccagct gacagaccag
     4921 ttcacctcct ttgccaagat gggcacccag tctggcttcc tgttttacgt gcctgcccca
     4981 tatacatcta agatcgatcc cctgaccggc ttcgtggacc ccttcgtgtg gaaaaccatc
     5041 aagaatcacg agagccgcaa gcacttcctg gagggcttcg actttctgca ctacgacgtg
     5101 aaaaccggcg acttcatcct gcactttaag atgaacagaa atctgtcctt ccagaggggc
     5161 ctgcccggct ttatgcctgc atgggatatc gtgttcgaga agaacgagac acagtttgac
     5221 gccaagggca cccctttcat cgccggcaag agaatcgtgc cagtgatcga gaatcacaga
     5281 ttcaccggca gataccggga cctgtatcct gccaacgagc tgatcgccct gctggaggag
     5341 aagggcatcg tgttcaggga tggctccaac atcctgccaa agctgctgga gaatgacgat
     5401 tctcacgcca tcgacacgat ggtggccctg atccgcagcg tgctgcagat gcggaactcc
     5461 aatgccgcca caggcgagga ctatatcaac agccccgtgc gcgatctgaa tggcgtgtgc
     5521 ttcgactccc ggtttcagaa cccagagtgg ccaatggacg ccgatgccaa tggcgcctac
     5581 cacatcgccc tgaagggcca gctgctgctg aatcacctga aggagagcaa ggatctgaag
     5641 ctgcagaacg gcatctccaa tcaggactgg ctggcctaca tccaggagct gcgcaacaaa
     5701 aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc ctacccatac
     5761 gatgttccag attacgctta tccctacgac gtgcctgatt atgcataccc atatgatgtc
     5821 cccgactatg ccggaagcga ggccagcggt tccggacggg ctgacgcatt ggacgatttt
     5881 gatctggata tgctgggaag tgacgccctc gatgattttg accttgacat gcttggttcg
     5941 gatgcccttg atgactttga cctcgacatg ctcggcagtg acgcccttga tgatttcgac
     6001 ctggacatgc tgattaactc tagaagttcc ggatctccga aaaagaaacg caaagttggt
     6061 agccagtacc tgcccgacac cgacgaccgg caccggatcg aggaaaagcg gaagcggacc
     6121 tacgagacat tcaagagcat catgaagaag tcccccttca gcggccccac cgaccctaga
     6181 cctccaccta gaagaatcgc cgtgcccagc agatccagcg ccagcgtgcc aaaacctgcc
     6241 ccccagcctt accccttcac cagcagcctg agcaccatca actacgacga gttccctacc
     6301 atggtgttcc ccagcggcca gatctctcag gcctctgctc tggctccagc ccctcctcag
     6361 gtgctgcctc aggctcctgc tcctgcacca gctccagcca tggtgtctgc actggctcag
     6421 gcaccagcac ccgtgcctgt gctggctcct ggacctccac aggctgtggc tccaccagcc
     6481 cctaaaccta cacaggccgg cgagggcaca ctgtctgaag ctctgctgca gctgcagttc
     6541 gacgacgagg atctgggagc cctgctggga aacagcaccg atcctgccgt gttcaccgac
     6601 ctggccagcg tggacaacag cgagttccag cagctgctga accagggcat ccctgtggcc
     6661 cctcacacca ccgagcccat gctgatggaa taccccgagg ccatcacccg gctcgtgaca
     6721 ggcgctcaga ggcctcctga tccagctcct gcccctctgg gagcaccagg cctgcctaat
     6781 ggactgctgt ctggcgacga ggacttcagc tctatcgccg atatggattt ctcagccttg
     6841 ctgggctctg gcagcggcag ccgggattcc agggaaggga tgtttttgcc gaagcctgag
     6901 gccggctccg ctattagtga cgtgtttgag ggccgcgagg tgtgccagcc aaaacgaatc
     6961 cggccatttc atcctccagg aagtccatgg gccaaccgcc cactccccgc cagcctcgca
     7021 ccaacaccaa ccggtccagt acatgagcca gtcgggtcac tgaccccggc accagtccct
     7081 cagccactgg atccagcgcc cgcagtgact cccgaggcca gtcacctgtt ggaggatccc
     7141 gatgaagaga cgagccaggc tgtcaaagcc cttcgggaga tggccgatac tgtgattccc
     7201 cagaaggaag aggctgcaat ctgtggccaa atggaccttt cccatccgcc cccaaggggc
     7261 catctggatg agctgacaac cacacttgag tccatgaccg aggatctgaa cctggactca
     7321 cccctgaccc cggaattgaa cgagattctg gataccttcc tgaacgacga gtgcctcttg
     7381 catgccatgc atatcagcac aggactgtcc atcttcgaca catctctgtt ttaagcggcc
     7441 gcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     7501 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     7561 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     7621 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     7681 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     7741 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     7801 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     7861 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     7921 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
     7981 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8041 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8101 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8161 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8221 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8281 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8341 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg
     8401 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8461 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa
     8521 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9181 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9541 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccaagcg cagaagtggt
     9721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9901 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10141 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10201 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10261 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10321 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10381 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg
//
