LOCUS       sequence-220565-        8699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite (attR3/attR4) Gateway vector for expression in oomycetes. 
            Confers resistance to geneticin (G418). Contains additional cassette
            for constitutive expression of mCitrine..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Evangelisti E, Shenhav L, Yunusov T, Le Naour-Vernet M, Rink P, 
            Schornack S
  TITLE     Hydrodynamic Shape Changes Underpin Nuclear Rerouting in Branched 
            Hyphae of an Oomycete Pathogen.
  JOURNAL   MBio. 2019 Oct 1;10(5). pii: mBio.01516-19. doi: 
            10.1128/mBio.01516-19.
  PUBMED    31575765
REFERENCE   2  (bases 1 to 8699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-10-1"; volume: "10(5). pii: mBio.01516-19. doi"; pages: 
            " 10.1128/mBio.01516-19"
COMMENT     Sequence Label: pTORKm43GWY
FEATURES             Location/Qualifiers
     source          1..8699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(322..341)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(323..341)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(348..365)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(348..364)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(357..379)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(505..960)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(642..663)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     854..873
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        987..1091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1092..1160,1161..1952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1310..1329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2123..2711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2612..2631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2865..2882
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2999..3020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3035..3040,3041..3058,3059..3065)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3073..3089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3078..3100
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3097..3113
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3097..3113
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3134..3152
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3745..3761
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3746..3762
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(3768..3770,3771..3773,3774..4484)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQGRTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3813..3834)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4074..4093)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4421..4442
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    5184..5307
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(5348..5653)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVFTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5450..5469)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(5973..6653)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     6568..6587
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(6707..6713,6714..6731,6732..6737))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(6762..6885)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7499..8293
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7553..7572)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8163..8182
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tacgccgcct tagtaacaaa catgccaaaa gaaggaaagc tttcaccagc tttcaagttt
       61 aatcaatctt tgagagtcac aattgcaaga ataaatgaat ttttacaaca cagttcgatg
      121 cactgtgttt cgtcttgatg aaaattggac ggcaaatcga gtatcttaaa caaatctcgg
      181 cataggctgt ccattttgtg gtcgcctctt tgttggtagt ttgcagtagt ttcattagta
      241 gaagatctgc atcctcaatc tgctaaacgt cttgagcagc tatgagacga tccggggaat
      301 tggggcccga gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
      361 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      421 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      481 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
      541 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
      601 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
      661 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
      721 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
      781 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
      841 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
      901 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
      961 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2881 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2941 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     3001 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     3061 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     3121 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtacct ctgatggaca
     3181 aagggtcgcc tctacgtacc cgcgacaatc tcggcttttc caatgtcgac agctggcata
     3241 tcgcacgcga ggttaggcgt cttcagccgg cgtaaccaca aacctcgagt caagtaccct
     3301 ttttcagacc ttaatttccg tgtccattcc ctcgcgttta cacgaaggtt tctgagaaat
     3361 accacatgtc acagagagag agaagattgg agaaccttct caacctgacc cgtgcagagc
     3421 gcttttgcgt cctaccatcc gttaattata gcaccaccac cacatgtctc gccggaactc
     3481 tcttatccgc tctttttgat cgtctttttt gcgccccatt aaagacatct cttgcacagg
     3541 gatcgtttct gattggccat ttcacttcct cttttacatc cgaaaaccac ctgtttgtgg
     3601 ggttgtgaag ccacaaccgg acgctcaaaa agcctcgccc gactcgccca cgatcggaag
     3661 gctcattctc cttttcactc tcacgtgccc aagtcccaac cgactctttt tcgaccttca
     3721 ctctcaccga caacgggccc cccctcgagg tcgacggtat cgataccatg gtgagcaagg
     3781 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3841 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3901 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     3961 tcggctacgg cctgatgtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     4021 tcaagtccgc catgcccgaa ggctacgtcc aggggcgcac catcttcttc aaggacgacg
     4081 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4141 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4201 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     4261 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     4321 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     4381 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     4441 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaggcc gaaggtcgag
     4501 ctgtgtgcac tgagtgcgaa gttgcagatt ttgtgtctat gcatttaata caatttcctt
     4561 ttttccattt tcgttgtttt ttttgtgaaa gaacgattgt tttcaaaaga aggccgtttg
     4621 tagaacaatt tttttttata ctagcatttg gtattcgagt gtgacgaatc ggtgatggcg
     4681 ctttacaaat gaaatataac gctgaacaag cgcagtgaca gctttatttt ataacagaga
     4741 ataaagacca aagtagagta gactgatggc ggcttgaaac aaaactacgt gacttctaat
     4801 gcaaagaaaa ccatacccca cgctacttag aattgtttct ctttggttga tgaccgctat
     4861 agtcgtatgg agtgatccct ttttgtcaat atttcgattc ttgctaaaac cgtgactatt
     4921 ttgaggcaac tgtgtgtact taacatggta gaaatttcta tgtttaagcc agctgacaat
     4981 cattctttat ttatcgtaaa ccaaccaggt accgggccca ttatacctcg tattcgatta
     5041 aatgatacaa ctcattgcca caccgcaaga cggcgtcacc accatagaca gcctttcaag
     5101 cacccccttg tctatgagct gccacgtcag ctgccgctcc taatatccac ccgcgtccct
     5161 attcccgtca tatgccaagc tatcaacttt gtatagaaaa gttgaacgag aaacgtaaaa
     5221 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     5281 taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt ataagggagc
     5341 ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca ttttcgcggt
     5401 gactgagatc agccacttct tccccgataa cggagaccgg cacactggcc atatcggtgg
     5461 tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca cgggggactt
     5521 tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa
     5581 taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta taggtgaaaa
     5641 ccttaaactg catttcacca gcccctgttc tcgtcggcaa aagagccgtt catttcaata
     5701 aaccgggcga cctcagccat cccttcttga ttttccgctt tccagcgttc ggcacgcaga
     5761 cgacgggctt cattctgcat ggttgtgctt accgaaccgg agatactgac atcatatatg
     5821 ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt gatacgctgc ttcatagcat
     5881 acctcttttt gacatacttc gggtatacat atcagtatat attcttatac cgcaaaaatc
     5941 agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatcctc tagattacgc
     6001 cccgcctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     6061 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     6121 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     6181 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     6241 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     6301 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     6361 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     6421 ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     6481 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     6541 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     6601 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     6661 tccttagctc ctgaaaatct cgacggatcc taactcaaaa tccacacatt atacgagccg
     6721 gaagcataaa gtgtaaagcc tggggtgcct aatgcggccg ccatagtgac tggatatgtt
     6781 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     6841 ttatatcatt ttacgtttct cgttcaactt tattatacat agttgataat tcacatatgc
     6901 tcaatgcaat tgactgtgtc ctcgattatt tctattattt aatattattt ccattaaaaa
     6961 agaaatccag ttcgtggcaa aataagacaa aggtttaaat aaaaatttat cgttattaaa
     7021 ctcgtaactt accttcttca tggctcaacc caatctgtat atcgacttat ttaatttggt
     7081 taacaaatcg gttttcgtcg caaatagggg atgacaacta aaataccaat ttgtgtcctt
     7141 gcgacacgtc aacaggtggc tgccaatatc tcaggttttg agcacacaac gctgtgattg
     7201 gctatctgta gatcgcttcg agaactttcg gaccgacata tcagaacgca gtcatgtcca
     7261 atccagctaa cgattggtcg aaaccgcact tcatcgcttc atttcaacca attcccatct
     7321 actaaaacga gcgtcggaat agtctcgaac gtaaccccac gtagtgggtg gcagcgcccc
     7381 tcgtatcttt caatcgccac tcgagtctca agtttgctca ctttgaaatt ttccatctgg
     7441 actacacatc aaatctaact caacttttat caattgaaac acaagggccc cgtttcgcat
     7501 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7561 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7621 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7681 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7741 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7801 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7861 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7921 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7981 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     8041 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8101 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8161 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8221 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8281 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     8341 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8401 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8461 caccccccgg atccccgggg tcgactagaa tacttagcaa ttggttagtt tgaaaattgt
     8521 caaagcagag ttttttcgac aataaaattt attttctaaa tatttgattt agcgttgact
     8581 taatttgttg attcggttgt catcttttgt gaagatgccc gagagtctag taaatctcaa
     8641 gcgaaacata gtgagaaggt cactatggct ggtttgcatg agtctgggcc tattgtgct
//
