LOCUS       sequence-220571-        5118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For mammalian expression of beta-actin with N-terminal Spot-Tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5118)
  TITLE     ChromoTek Spot-tag plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSpot-Tag-Actin
FEATURES             Location/Qualifiers
     source          1..5118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             616..651
                     /codon_start=1
                     /product="engineered Spot-Tag(R) peptide that binds with 
                     high affinity to a nanobody"
                     /label=Spot-Tag
                     /translation="PDRVRAVSHWSS"
     polyA_signal    1906..2027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1943..1962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1997..2016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2034..2489)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2171..2192)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2383..2402
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2516..2620
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2622..2979
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2622..2813
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2645..2665)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2830..2965
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3014..3808
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3014..3808
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3068..3087)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3678..3697
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3996..4015)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4040..4087
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4416..5004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4905..4924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatgcctga tcgcgtgcgc gccgtgagcc attggagcag cgatgatgat
      661 atcgccgcgc tcgtcgtcga caacggctcc ggcatgtgca aggccggctt cgcgggcgac
      721 gatgcccccc gggccgtctt cccctccatc gtggggcgcc ccaggcacca gggcgtgatg
      781 gtgggcatgg gtcagaagga ttcctatgtg ggcgacgagg cccagagcaa gagaggcatc
      841 ctcaccctga agtaccccat cgagcacggc atcgtcacca actgggacga catggagaaa
      901 atctggcacc acaccttcta caatgagctg cgtgtggctc ccgaggagca ccccgtgctg
      961 ctgaccgagg cccccctgaa ccccaaggcc aaccgcgaga agatgaccca gatcatgttt
     1021 gagaccttca acaccccagc catgtacgtt gctatccagg ctgtgctatc cctgtacgcc
     1081 tctggccgta ccactggcat cgtgatggac tccggtgacg gggtcaccca cactgtgccc
     1141 atctacgagg ggtatgccct cccccatgcc atcctgcgtc tggacctggc tggccgggac
     1201 ctgactgact acctcatgaa gatcctcacc gagcgcggct acagcttcac caccacggcc
     1261 gagcgggaaa tcgtgcgtga cattaaggag aagctgtgct acgtcgccct ggacttcgag
     1321 caagagatgg ccacggctgc ttccagctcc tccctggaga agagctacga gctgcctgac
     1381 ggccaggtca tcaccattgg caatgagcgg ttccgctgcc ctgaggcact cttccagcct
     1441 tccttcctgg gcatggagtc ctgtggcatc cacgaaacta ccttcaactc catcatgaag
     1501 tgtgacgtgg acatccgcaa agacctgtac gccaacacag tgctgtctgg cggcaccacc
     1561 atgtaccctg gcattgccga caggatgcag aaggagatca ctgccctggc acccagcaca
     1621 atgaagatca agatcattgc tcctcctgag cgcaagtact ccgtgtggat cggcggctcc
     1681 atcctggcct cgctgtccac cttccagcag atgtggatca gcaagcagga gtatgacgag
     1741 tccggcccct ccatcgtcca ccgcaaatgc ttctagggat ccaccggatc tagataactg
     1801 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     1861 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     1921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt
     2041 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     2101 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     2161 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     2221 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     2281 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     2341 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     2401 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     2461 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     2521 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2581 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     2641 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     2701 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     2761 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     2821 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     2881 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     2941 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     3001 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     3061 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     3121 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     3181 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     3241 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     3301 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     3361 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     3421 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     3481 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     3541 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     3601 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     3661 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     3721 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     3781 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     3841 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     3901 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     3961 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     4021 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     4081 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     4141 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     4201 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     4261 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4321 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4381 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4441 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4501 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4561 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4621 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4681 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4741 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4801 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4861 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4921 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4981 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5041 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5101 cgtattaccg ccatgcat
//
