LOCUS       sequence-220584-        9466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Accessory plasmid with inducible TEV protease and unnatural tRNA 
            system (AcF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Cambray G, Guimaraes JC, Arkin AP
  TITLE     Evaluation of 244,000 synthetic sequences reveals design principles 
            to optimize translation in Escherichia coli.
  JOURNAL   Nat Biotechnol. 2018 Nov;36(10):1005-1015. doi: 10.1038/nbt.4238. 
            Epub 2018 Sep 24.
  PUBMED    30247489
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.4238"; 
            journalName: "Nat Biotechnol"; date: "2018-11"; volume: "36"; issue:
            "10"; pages: "1005-1015"
COMMENT     Sequence Label: pGC4593
FEATURES             Location/Qualifiers
     source          1..9466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(19..113)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(134..793)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     708..727
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(794..896)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(934..1560)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     1465..1484
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        1576..1631
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    1612..1630
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             1643..1654
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1664..2371
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMDKPE
                     EPFQPVKEATQLMN"
     terminator      2410..2481
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2497..2524
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      3245..4016
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             4017..4679
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             complement(5293..6171)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPSDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        6198..6482
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     6405..6424
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             7449..7466
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7522..7539)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 gtcatgacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
       61 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
      121 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
      181 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
      241 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
      301 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
      361 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
      421 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
      481 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
      541 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
      601 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
      661 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
      721 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
      781 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
      841 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
      901 ctcattttcg ccagatatcg acgcggccgc gtcttaagac ccactttcac atttaagttg
      961 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
     1021 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
     1081 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
     1141 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
     1201 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
     1261 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
     1321 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
     1381 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
     1441 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
     1501 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
     1561 taattcctaa tttttgttga cactctatcg ttgatagagt tattttacca ctccctatca
     1621 gtgatagaga aaagaattca ttaaagagga gaaaggtaac atgggagaaa gcttgtttaa
     1681 ggggccgcgt gattacaacc cgatatcgag caccatttgt catttgacga atgaatctga
     1741 tgggcacaca acatcgttgt atggtattgg atttgggccc ttcatcatta caaacaagca
     1801 cttgtttaga agaaataatg gaacactgtt ggtccaatca ctacatggtg tattcaaggt
     1861 caagaacacc acgactttgc aacaacacct catcgatggg cgggacatga tcattattcg
     1921 aatgcctaag gatttcccac catttcctca aaagctgaaa tttcgcgagc cacaacggga
     1981 agagcgcatt tgtcttgtga caaccaactt ccaaactaag agcatgtcta gcatggtgtc
     2041 agacactagt tgcacattcc cttcatctga tggcatattc tggaagcatt ggattcaaac
     2101 caaggatggg cagtgtggca gtccattagt atcaactcga gatgggttca ttgttggtat
     2161 acactcagca tcgaatttca ccaacacaaa caattatttc acaagcgtgc cgaaaaactt
     2221 catggaattg ttgacaaatc aggaggcgca gcagtgggtt agtggttggc gattaaatgc
     2281 tgactcagta ttgtgggggg gccataaagt tttcatggac aaacctgaag agccttttca
     2341 gccagttaag gaagcgactc aactcatgaa ttaataagga tccaaactcg agtaaggatc
     2401 tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2461 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2521 tgcgtttata cctgctagcg cggccgcaaa cgtggtctgg tcgcgggtca tctgcgcttt
     2581 gtttccgatg aactccttgg ccgacagcct gccgtcctgc gtcagcggca ccacgaacgc
     2641 ggtcatgtgc gggctggttt cgtcacggtg gatgctggcc gtcacgatgc gatccgcccc
     2701 gtacttgtcc gccagccact tgtgcgcctt ctcgaagaac gccgcctgct gttcttggct
     2761 ggccgacttc caccattccg ggctggccgt catgacgtac tcgaccgcca acacagcgtc
     2821 cttgcgccgc ttctctggca gcaactcgcg cagtcggccc atcgcttcat cggtgctgct
     2881 ggccgcccag tgctcgttct ctggcgtcct gctggcgtca gcgttgggcg tctcgcgctc
     2941 gcggtaggcg tgcttgagac tggccgccac gttgcccatt ttcgccagct tcttgcatcg
     3001 catgatcgcg tatgccgcca tgcctgcccc tcccttttgg tgtccaaccg gctcgacggg
     3061 ggcagcgcaa ggcggtgcct ccggcgggcc actcaatgct tgagtatact cactagactt
     3121 tgcttcgcaa agtcgtgacc gcctacggcg gctgcggcgc cctacgggct tgctctccgg
     3181 gcttcgccct gcgcggtcgc tgcgctccct tgccagccca tggatatgtg gacgatggcc
     3241 gcgagcggcc accggctggc tcgcttcgct cggcccgtgg acaaccctgc tggacaagct
     3301 gatggacagg ctgcgcctgc ccacgagctt gaccacaggg attgcccacc ggctacccag
     3361 ccttcgacca catacccacc ggctccaact gcgcggcctg cggccttgcc ccatcaattt
     3421 ttttaatttt ctctggggaa aagcctccgg cctgcggcct gcgcgcttcg cttgccagtt
     3481 ggacaccaag tggaaggcgg gtcaaggctc gcgcagcgac cgcgcagcgg cttggccttg
     3541 acgcgcctgg aacgacccaa gcctatgcga gtgggggcag tcgaagggcg aagcccgccc
     3601 gcctgccccc cgagcctcac ggcggcgagt gcgggggttc caagggggca gcgccacctt
     3661 gggcaaggcc gaaggccgcg cagtcgatca acaagccccg gaggggccac tttttgccgg
     3721 agggggagcc gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca
     3781 aagaactaga tatagggcga aatgcgaaag acttaaaaat caacaactta aaaaaggggg
     3841 gtacgcaaca gctcattgcg gcaccccccg caatagctca ttgcgtaggt taaagaaaat
     3901 ctgtaattga ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc gaaaagttgc
     3961 agctgattgc gcatggtgcc gcaaccgtgc ggcaccccta ccgcatggag ataagcatgg
     4021 ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa gcccggtcac tgggtgcaaa
     4081 cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc gaggaaaccc acggcggcaa
     4141 tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa cgccgtggtg gtcagccaga
     4201 agacactttc caagctcatc ggacgttctt tgcggacggt ccaatacgca gtcaaggact
     4261 tggtggccga gcgctggatc tccgtcgtga agctcaacgg ccccggcacc gtgtcggcct
     4321 acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga ccagttgcgc ctgtcggtgt
     4381 tcagtgccgc cgtggtggtt gatcacgacg accaggacga atcgctgttg gggcatggcg
     4441 acctgcgccg catcccgacc ctgtatccgg gcgagcagca actaccgacc ggccccggcg
     4501 aggagccgcc cagccagccc ggcattccgg gcatggaacc agacctgcca gccttgaccg
     4561 aaacggagga atgggaacgg cgcgggcagc agcgcctgcc gatgcccgat gagccgtgtt
     4621 ttctggacga tggcgagccg ttggagccgc cgacacgggt cacgctgccg cgccggtagc
     4681 acttgggttg cgcagcaacc cgtaagtgcg ctgttccaga ctatcggctg tagccgcctc
     4741 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     4801 tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccgttttt atcaggctct
     4861 gggaggcaga ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa
     4921 actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg
     4981 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt
     5041 cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca
     5101 accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc
     5161 atcgcagtac ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5221 ttaacaaaat attaacgctt acaatttcca ttcgccattc aggctgcgca actgttgaag
     5281 cttggtaccc aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc
     5341 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg
     5401 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
     5461 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
     5521 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc
     5581 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg
     5641 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
     5701 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
     5761 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc
     5821 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
     5881 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc
     5941 tcctggcggg aacagcaaaa tatcactcgg tcggcaaaca aattctcgtc cctgattttt
     6001 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
     6061 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
     6121 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat
     6181 actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg
     6241 cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct
     6301 gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg
     6361 cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt
     6421 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc
     6481 atacccgttt ttttgggcta acaggaggaa ttagatctat ggacgaattt gaaatgataa
     6541 agagaaacac atctgaaatt atcagcgagg aagagttaag agaggtttta aaaaaagatg
     6601 aaaaatctgc tctgataggt tttgaaccaa gtggtaaaat acatttaggg cattatctcc
     6661 aaataaaaaa gatgattgat ttacaaaatg ctggatttga tataattata ttgttggctg
     6721 atttacacgc ctatttaaac cagaaaggag agttggatga gattagaaaa ataggagatt
     6781 ataacaaaaa agtttttgaa gcaatggggt taaaggcaaa atatgtttat ggaagtgaat
     6841 tccagcttga taaggattat acactgaatg tctatagatt ggctttaaaa actaccttaa
     6901 aaagagcaag aaggagtatg gaacttatag caagagagga tgaaaatcca aaggttgctg
     6961 aagttatcta tccaataatg caggttaatg gttgtcatta taggggcgtt gatgttgctg
     7021 ttggagggat ggagcagaga aaaatacaca tgttagcaag ggagctttta ccaaaaaagg
     7081 ttgtttgtat tcacaaccct gtcttaacgg gtttggatgg agaaggaaag atgagttctt
     7141 caaaagggaa ttttatagct gttgatgact ctccagaaga gattagggct aagataaaga
     7201 aagcatactg cccagctgga gttgttgaag gaaatccaat aatggagata gctaaatact
     7261 tccttgaata tcctttaacc ataaaaaggc cagaaaaatt tggtggagat ttgacagtta
     7321 atagctatga ggagttagag agtttattta aaaataagga attgcatcca atgcgcttaa
     7381 aaaatgctgt agctgaagaa cttataaaga ttttagagcc aattagaaag agattataat
     7441 aagtcgacca tcatcatcat catcattgag tttaaacggt ctccagcttg gctgttttgg
     7501 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     7561 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     7621 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     7681 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttg tttgtgagct
     7741 cccggtcatc aatcatcccc ataatccttg ttagattatc aattttaaaa aactaacagt
     7801 tgtcagcctg tcccgcttta atatcatacg ccgttatacg ttgtttacgc tttgaggaat
     7861 cccatatgga cgaatttgaa atgataaaga gaaacacatc tgaaattatc agcgaggaag
     7921 agttaagaga ggttttaaaa aaagatgaaa aatctgctct gataggtttt gaaccaagtg
     7981 gtaaaataca tttagggcat tatctccaaa taaaaaagat gattgattta caaaatgctg
     8041 gatttgatat aattatattg ttggctgatt tacacgccta tttaaaccag aaaggagagt
     8101 tggatgagat tagaaaaata ggagattata acaaaaaagt ttttgaagca atggggttaa
     8161 aggcaaaata tgtttatgga agtgaattcc agcttgataa ggattataca ctgaatgtct
     8221 atagattggc tttaaaaact accttaaaaa gagcaagaag gagtatggaa cttatagcaa
     8281 gagaggatga aaatccaaag gttgctgaag ttatctatcc aataatgcag gttaatggtt
     8341 gtcattatag gggcgttgat gttgctgttg gagggatgga gcagagaaaa atacacatgt
     8401 tagcaaggga gcttttacca aaaaaggttg tttgtattca caaccctgtc ttaacgggtt
     8461 tggatggaga aggaaagatg agttcttcaa aagggaattt tatagctgtt gatgactctc
     8521 cagaagagat tagggctaag ataaagaaag catactgccc agctggagtt gttgaaggaa
     8581 atccaataat ggagatagct aaatacttcc ttgaatatcc tttaaccata aaaaggccag
     8641 aaaaatttgg tggagatttg acagttaata gctatgagga gttagagagt ttatttaaaa
     8701 ataaggaatt gcatccaatg cgcttaaaaa atgctgtagc tgaagaactt ataaagattt
     8761 tagagccaat tagaaagaga ttataactgc agtttcaaac gctaaattgc ctgatgcgct
     8821 acgcttatca ggcctacatg atctctgcaa tatattgagt ttgcgtgctt ttgtaggccg
     8881 gataaggcgt tcacgccgca tccggcaaga aacagcaaac aatccaaaac gccgcgttca
     8941 gcggcgtttt ttctgctttt cttcgcgaat taattccgct tcgcaacatg tgagcaccgg
     9001 tttattgact accggaagca gtgtgaccgt gtgcttctca aatgcctgag gccagtttgc
     9061 tcaggctctc cccgtggagg taataattga cgatatgatc agtgcacggc taactaagcg
     9121 gcctgctgac tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt
     9181 atctgcgcag taagatgcgc cccgcattcc ggcggtagtt cagcagggca gaacggcgga
     9241 ctctaaatcc gcatggcagg ggttcaaatc ccctccgccg gaccaaattc gaaaagcctg
     9301 ctcaacgagc aggctttttt gcatgctcga gcagctcagg gtcgaatttg ctttcgaatt
     9361 tctgccattc atccgcttat tatcacttat tcaggcgtag caaccaggcg tttaagggca
     9421 ccaataactg ccttaaaaaa attacgcccc gccctgccac tctaga
//
