LOCUS       sequence-220591-       12562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the E2F3 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12562)
  AUTHORS   Rady B, Chen Y, Vaca P, Wang Q, Wang Y, Salmon P, Oberholzer J
  TITLE     Overexpression of E2F3 promotes proliferation of functional human 
            beta cells without induction of apoptosis.
  JOURNAL   Cell Cycle. 2013 Aug 15;12(16):2691-702. doi: 10.4161/cc.25834. Epub
            2013 Jul 29.
  PUBMED    23907129
REFERENCE   2  (bases 1 to 12562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/cc.25834"; 
            journalName: "Cell Cycle"; date: "2013-08-15- 15"; volume: "12"; 
            issue: "16"; pages: "2691-702"
COMMENT     Sequence Label: pWPI-E2F3
FEATURES             Location/Qualifiers
     source          1..12562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1038..1062)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1086..1653
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1104..1655
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1248..1265)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1475..1494
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1658..1660,1661..1663,1664..2374)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1658..1660,1661..1663,1664..2374)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1703..1724)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1964..1983)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2311..2332
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2453..2469)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2454..2470)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    2483..3071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2536..2556)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2954..2965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3215..3248)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             3268..3448
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3473..3491)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3474..3491)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3781..3800)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4014..4033)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4133..4155
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4193..4211)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4279..4383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4384..4452,4453..5244)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4602..4621)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5415..6003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5904..5923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6157..6174
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6244..6264)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6249..6578
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6429..6564
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7712..7777
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7907..7927
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8352..8486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8389..8408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8443..8462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8654..9287
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9334..9459
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9952..10185
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10370..10414
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10797..11975
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          11028..11966
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     11923..11943
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    12023..12140
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    12172..12196
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcggcggcag cggcggcggc ggcggccctc cggcaaagcg aaggctggag ctaggagaaa
       61 gcggtcatca gtacctctca gatggtttaa aaacccccaa gggcaaagga agagctgcac
      121 tacgaagtcc agatagtcca aaaactccaa aatctccctc agaaaaaacg cggtatgata
      181 cgtctcttgg tctgctcacc aagaagttca ttcagctcct gagccagtca cccgatgggg
      241 tattggattt gaacaaggca gcagaagtgc taaaagtgca aaagagaagg atttatgata
      301 tcaccaacgt tctggaaggc atccacctca ttaagaagaa gtctaaaaac aacgtccaat
      361 ggatgggctg cagtctgtct gaggatgggg gcatgctggc ccagtgtcaa ggcctgtcaa
      421 aagaagtgac cgagctcagt caggaagaga agaaattaga tgaactgatc caaagctgca
      481 ccctggacct caaactgtta accgaggatt cagagaatca aaggttagct tatgttacat
      541 atcaagatat tcgaaaaatt agtggcctta aagaccaaac tgttatagtt gtgaaagccc
      601 ctccagaaac aagacttgaa gtgcctgact caatagagag cctacaaata catttggcaa
      661 gtacccaagg gcccattgag gtttacttat gtccagaaga gactgaaaca cacagtccaa
      721 tgaaaacaaa caaccaagac cacaatggga atatccctaa acccgcttcc aaagacttgg
      781 cttcaaccaa ctcaggacat agcgattgct cagtttctat gggaaacctt tctcctctgg
      841 cctccccagc caacctctta cagcagactg aggaccaaat tccttccaac ctagaaggac
      901 cgtttgtgaa cttactgcct cccctgctgc aagaggacta tctcctgagc ctcggggagg
      961 aggaaggcat cagcgatctc ttcgatgctt acgatttgga aaagctccca ctggtggaag
     1021 acttcatgtg tagttgaacc cagctttctt gtacaaagtg gtgataaact acgggctgca
     1081 ggaattccgc cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     1141 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     1201 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     1261 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     1321 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     1381 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     1441 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     1501 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     1561 cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc gaaccacggg
     1621 gacgtggttt tcctttgaaa aacacgatga taataccatg gtgagcaagg gcgaggagct
     1681 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1741 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1801 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1861 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1921 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1981 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2041 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2101 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2161 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2221 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     2281 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2341 cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc gactagctag
     2401 tagctagcta gctagtcgag ctcaagcttc gaattcgata tcaagcttat cgcgataccg
     2461 tcgacctcga gggaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac
     2521 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2581 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     2641 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     2701 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     2761 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     2821 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     2881 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     2941 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3001 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3061 cgcctccccg catcgggaat tcgagctcgg tacctttaag accaatgact tacaaggcag
     3121 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     3181 aacgaagaca agatgggatc aattcaccat gggaataact tcgtatagca tacattatac
     3241 gaagttatgc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     3301 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3361 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3421 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
     3481 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     3541 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
     3601 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
     3661 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
     3721 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     3781 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     3841 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     3901 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     3961 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
     4021 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     4081 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     4141 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     4201 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4261 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4381 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4441 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4501 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4561 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4621 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4681 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     4741 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     4801 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     4861 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     4921 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     4981 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5041 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5101 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5161 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5221 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5281 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5341 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5401 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5461 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5521 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5581 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5641 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5701 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5761 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5821 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5881 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5941 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6001 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6061 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6121 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6181 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6241 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6301 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6361 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6421 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6481 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6541 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     6601 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     6661 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     6721 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     6781 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     6841 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
     6901 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     6961 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
     7021 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     7081 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     7141 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     7201 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     7261 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     7321 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     7381 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     7441 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     7501 cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc
     7561 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt
     7621 tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     7681 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     7741 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     7801 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     7861 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     7921 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     7981 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     8041 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     8101 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     8161 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     8221 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     8281 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     8341 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8401 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8461 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     8521 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     8581 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     8641 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     8701 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     8761 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     8821 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     8881 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     8941 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     9001 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     9061 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     9121 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9181 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9241 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     9301 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     9361 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     9421 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     9481 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     9541 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     9601 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     9661 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     9721 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     9781 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     9841 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9901 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9961 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    10021 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    10081 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    10141 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    10201 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    10261 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    10321 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    10381 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    10441 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    10501 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    10561 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    10621 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg
    10681 agactagcct cgaccatcga tgtcgacgat aagctttgca aagatggata aagttttaaa
    10741 cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct
    10801 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
    10861 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
    10921 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
    10981 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
    11041 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
    11101 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
    11161 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
    11221 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
    11281 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
    11341 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
    11401 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
    11461 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
    11521 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
    11581 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
    11641 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
    11701 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
    11761 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
    11821 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
    11881 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
    11941 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt
    12001 aatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag
    12061 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    12121 ttacaaaaat tcaaaatttt atcgatcacg agactagcct cgaggtttat cacaagtttg
    12181 tacaaaaaag caggcttcac catgagaaag ggaatccagc ccgctctgga gcagtacctg
    12241 gtgaccgccg ggggtgggga gggggcggct gtcgtcgccg ccgccgctgc agcctccatg
    12301 gacaaaaggg cactgctagc cagccccggc ttcgccgccg ccgccgccgc tgccgccgcc
    12361 ccgggcgcgt acatccagat cctcaccacg aacacttcca ccacctcctg ttcctcctcc
    12421 ctccaaagcg gcgccgtagc cgccggcccc ctcctcccca gtgcccccgg cgcggagcag
    12481 accgccggca gcctcctcta caccacgccg cacggaccct ccagcagagc cgggctgctg
    12541 cagcagccac cagcgctggg ac
//
