LOCUS       sequence-220592-       12478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the E2F2 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12478)
  AUTHORS   Rady B, Chen Y, Vaca P, Wang Q, Wang Y, Salmon P, Oberholzer J
  TITLE     Overexpression of E2F3 promotes proliferation of functional human 
            beta cells without induction of apoptosis.
  JOURNAL   Cell Cycle. 2013 Aug 15;12(16):2691-702. doi: 10.4161/cc.25834. Epub
            2013 Jul 29.
  PUBMED    23907129
REFERENCE   2  (bases 1 to 12478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/cc.25834"; 
            journalName: "Cell Cycle"; date: "2013-08-15- 15"; volume: "12"; 
            issue: "16"; pages: "2691-702"
COMMENT     Sequence Label: pWPI-E2F2
FEATURES             Location/Qualifiers
     source          1..12478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        117..1295
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          348..1286
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1243..1263
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1343..1460
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1492..1516
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2836..2860)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2884..3451
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2902..3453
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3046..3063)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3273..3292
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3456..3458,3459..3461,3462..4172)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3456..3458,3459..3461,3462..4172)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3501..3522)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3762..3781)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4109..4130
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4251..4267)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4252..4268)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    4281..4869
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4334..4354)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4752..4763)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5013..5046)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5066..5246
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5271..5289)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5272..5289)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5579..5598)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5812..5831)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5931..5953
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5991..6009)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6077..6181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6182..6250,6251..7042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6400..6419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7213..7801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7702..7721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7955..7972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8042..8062)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8047..8376
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8227..8362
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9510..9575
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9705..9725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10150..10284
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10187..10206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10241..10260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10452..11085
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11132..11257
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11750..11983
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12168..12212
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agactagcct cgaccatcga tgtcgacgat aagctttgca aagatggata aagttttaaa
       61 cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct
      121 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
      181 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
      241 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
      301 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
      361 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
      421 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
      481 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      541 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      601 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      661 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
      721 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      781 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
      841 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
      901 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
      961 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     1021 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     1081 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1141 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1201 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1261 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt
     1321 aatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag
     1381 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1441 ttacaaaaat tcaaaatttt atcgatcacg agactagcct cgaggtttat cacaagtttg
     1501 tacaaaaaag caggcttcac catgctgcaa gggccccggg ccttggcttc ggccgctggg
     1561 cagaccccga aggtggtgcc cgcgatgagc cccacagagc tgtggccatc cggcctcagc
     1621 agcccccagc tctgcccagc tactgctacc tactacacac cgctgtaccc gcagacggcg
     1681 cctcccgcag cggcgccagg cacctgcctc gacgccactc cccacggacc cgagggccaa
     1741 gttgtgcgat gcctgccggc aggccggctg ccggccaaaa ggaagctgga tctggagggg
     1801 attgggaggc ccgtcgtccc tgagttccca acccccaagg ggaagtgcat cagagtggat
     1861 ggcctcccca gccccaaaac ccccaaatcc cccggggaga agactcggta tgacacttcg
     1921 ctggggctgc tcaccaagaa gttcatttac ctcctgagcg agtcagagga tggggtcctg
     1981 gacctgaact gggccgctga ggtgctggac gtgcagaagc ggcgcatcta tgacatcacc
     2041 aacgtgctgg aaggcatcca gctcatccgc aagaaggcca agaacaacat ccagtgggta
     2101 ggcaggggaa tgtttgaaga ccccaccaga cctgggaagc agcaacagct ggggcaggag
     2161 ctgaaggagc tgatgaacac ggagcaggcc ttggaccatc tcatccagag ctgctctctg
     2221 agcttcaagc acctgactga ggacaaggcc aacaagaggc tggcctatgt gacttaccag
     2281 gatatccgtg ctgttggcaa ctttaaggag cagacagtga ttgccgtcaa ggcccctccg
     2341 cagacgagac tggaagtgcc cgacaggact gaggacaacc tgcagatata tctcaagagc
     2401 acccaagggc ccatcgaagt ctacctgtgc ccagaggagg tgcaggagcc ggacagtcct
     2461 tccgaggagc ctctcccctc tacctccacc ctctgcccca gccctgactc tgcccagccc
     2521 agcagcagca ccgaccctag catcatggag cccacagcat cctcagtgcc agcaccagcg
     2581 ccaacccccc agcaggcccc accgcctcca tccctggtcc ccttggaggc tactgacagc
     2641 ctgctggagc tgccgcaccc actcctgcag cagactgagg accagttcct gtccccgacc
     2701 ctggcgtgca gctcccctct gatcagcttc tccccatcct tggaccagga cgactacctg
     2761 tggggcttgg aggcgggtga gggcatcagc gatctcttcg actcctacga ccttggggac
     2821 ctgttgatta attgaaccca gctttcttgt acaaagtggt gataaactac gggctgcagg
     2881 aattccgccc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     2941 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     3001 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     3061 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     3121 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     3181 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     3241 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     3301 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     3361 catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga
     3421 cgtggttttc ctttgaaaaa cacgatgata ataccatggt gagcaagggc gaggagctgt
     3481 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     3541 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     3601 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     3661 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     3721 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     3781 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     3841 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     3901 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     3961 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4021 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     4081 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     4141 ggatcactct cggcatggac gagctgtaca agtccggact cagatctcga ctagctagta
     4201 gctagctagc tagtcgagct caagcttcga attcgatatc aagcttatcg cgataccgtc
     4261 gacctcgagg gaattccgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     4321 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4381 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4441 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4501 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4561 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4621 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     4681 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4741 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4801 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4861 cctccccgca tcgggaattc gagctcggta cctttaagac caatgactta caaggcagct
     4921 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4981 cgaagacaag atgggatcaa ttcaccatgg gaataacttc gtatagcata cattatacga
     5041 agttatgctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5101 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5161 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5221 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     5281 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     5341 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     5401 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     5461 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     5521 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     5581 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     5641 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     5701 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     5761 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     5821 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     5881 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5941 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     6001 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     6061 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6121 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6181 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6241 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6301 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6361 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6421 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6481 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6541 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6601 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6661 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6721 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6781 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6841 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6901 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6961 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7021 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7081 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7141 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7321 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7801 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7861 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7921 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7981 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8041 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8101 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8161 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8221 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8281 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8341 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
     8401 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     8461 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     8521 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     8581 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     8641 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
     8701 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     8761 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     8821 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     8881 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
     8941 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
     9001 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     9061 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
     9121 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
     9181 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
     9241 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
     9301 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
     9361 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
     9421 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     9481 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     9541 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     9601 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     9661 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     9721 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     9781 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     9841 tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata
     9901 atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact
     9961 atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt
    10021 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
    10081 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
    10141 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10201 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10261 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
    10321 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
    10381 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    10441 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
    10501 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
    10561 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
    10621 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
    10681 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
    10741 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
    10801 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
    10861 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
    10921 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
    10981 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    11041 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
    11101 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
    11161 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
    11221 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
    11281 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
    11341 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
    11401 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
    11461 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
    11521 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
    11581 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
    11641 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    11701 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    11761 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    11821 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    11881 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    11941 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    12001 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    12061 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    12121 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    12181 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    12241 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    12301 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    12361 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
    12421 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacg
//
