LOCUS       sequence-220608-        9537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-293
FEATURES             Location/Qualifiers
     source          1..9537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..65
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     149..168
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        149..167
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    168..192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             246..263
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3811..3900
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3913..4002
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(4620..4638)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4634..4681
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4718..5173
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4805..4824)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5015..5036
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5265..6080)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5265..6080)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5988..6007
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6072..6091
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6202..6790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6691..6710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6944..6961
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6976..7115
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7041..7060)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7201..7223
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7217..7408)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8217..9299)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9261..9280
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9300..9377)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
       61 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
      121 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
      181 gataacaatt cccctctaga aataattttg ccttaacttt aagaaggaga tataccatgg
      241 gcagccacca ccaccaccac cacagcggaa tgcagatctt tgttaagact ctcaccggaa
      301 agacaatcac cctagaggtg gaaagctccg acaccatcga caacgttaag gccaagatcc
      361 aggataagga gggcattcct ccggatcagc agaggcttat tttcgccggc aagcagctag
      421 aggatggccg tacgttggct gattacaata tccagaagga atccaccctc cacttggtcc
      481 tcaggctccg tggtggttaa gcttgcaatt aactttaaga aggagatata caatgcttca
      541 caagcgagct agtgaagcta acgataagaa cgataatacc atcatcggtt ccgatttagc
      601 atcatctaag aaacgccgta tcgatttcac tgaatcttcg tctgataagt catcttcgat
      661 tttagcttct ggtagtagca ggggttttca cggcgatagc gtcgttcagc aaatcgacat
      721 ggcttttggt aattcgaacc gtcaggagat tgatgaagat ctgcacagtc gccagctcgc
      781 cgtctatggt cgtgagacta tgaggcgtct ctttgcttcc aatgttctca tctcggggat
      841 gcacggtctt ggtgccgaga ttgccaagaa tcttatactt gctggtgtga agtctgtgac
      901 cttgcatgat gaaagagttg tagagctatg ggacttatca agcaactttg ttttctcgga
      961 ggatgatgtt ggcaagaata gagcagatgc ttctgttcag aagttacagg atcttaacaa
     1021 tgctgtggtt gtctctagct tgaccaaaag cttaaacaaa gaggatcttt ctggcttcca
     1081 ggttgttgta ttctctgaca taagcatgga aagagcaatc gaatttgatg actattgtca
     1141 cagccatcag cctcctatcg cttttgtcaa ggctgatgtc aggggtcttt tcggctctgt
     1201 cttttgtgat tttgggcctg aatttgcggt tcttgatgtt gatggagagg aaccccatac
     1261 aggcatcatt gcgtctatct ctaatgagaa ccaggcattt atttcctgtg tcgacgatga
     1321 gaggcttgag tttgaagatg gtgacctagt tgttttctca gaagttgagg gtatgacaga
     1381 attgaatgat gggaaaccaa ggaagataaa aagtacacgg ccatattcat tcacccttga
     1441 tgaggacact actaactatg gaacatatgt gaagggtggt attgtcactc aagtgaaaca
     1501 gccaaagtta ctgaatttca agccattgag ggaagcgctt aaggatccag gggattttct
     1561 atttagtgat ttctctaagt ttgatcggcc tccactcctt cacttagcat tccaggcact
     1621 tgatcatttt aaagctgaag ctggcagatt tcctgttgct ggctcagaag aggacgctca
     1681 gaagcttata tcgattgcca cagccatcaa taccgggcag ggtgatttaa aggtggagaa
     1741 tgttgatcag aagcttctaa gacacttttc ctttggagcc aaggctgtcc tgaatcctat
     1801 ggctgcaatg tttggcggta ttgttggaca ggaggttgtt aaagcttgct ctggaaaatt
     1861 tcatcctctc ttccagtttt tctactttga ttcagtggag tcactcccct ctgaacctgt
     1921 ggactcgagt gactttgcac caagaaacag ccggtatgat gcccaaatat ctgtatttgg
     1981 ggccaagttc cagaagaaac ttgaagatgc taaagttttc acagtagggt ctggtgctct
     2041 tggctgtgag ttcttgaaaa atttggctct gatgggggtt tcttgtggaa gccaagggaa
     2101 gctgacagtg acagatgatg atatcattga gaagagtaac ctcagtcgtc aatttctgtt
     2161 ccgtgactgg aacattggac aggctaaatc cacagttgct gcttcggctg ctgctgttat
     2221 aaatcctcgc ttcaacattg aggccctgca aaaccgtgtt ggcgcggaga ctgagaatgt
     2281 atttgacgat gccttctggg agaacttaac tgttgtcgtc aatgcactgg ataatgttaa
     2341 tgcgaggctc tatgttgatt cgaggtgctt gtatttccag aagcctctcc ttgagtctgg
     2401 aactcttggt acaaagtgca acacacagtc ggttatccca catctaactg aaaattacgg
     2461 tgcctcaagg gacccaccag agaaacaggc ccccatgtgt acagtgcatt ccttcccgca
     2521 taacattgat cactgtttaa cttgggctcg ctctgagttt gagggtctgc tggagaaaac
     2581 tcccgctgaa gtgaatgcat atctttctag cccagttgag tacactaact caatgatgag
     2641 tgctggtgat gctcaggcga gggacacatt ggagaggatt gttgagtgcc ttgaaaagga
     2701 gaagtgtgaa accttccaag actgcttaac ctgggcacga ctgaggtttg aggattactt
     2761 tgtgaaccgt gtcaagcaat tgatatacac atttcctgaa gatgctgcga ctagtaccgg
     2821 agctccattc tggtctgctc caaaaagatt ccctcgtcca ctccagtact cgtcgtccga
     2881 cccaagtctc cttaacttta tcacagcgac tgctatttta cgagcagaga catttgggat
     2941 cccgatacct gagtggacta agaacccaaa ggaagcagca gaagctgttg acagagtgat
     3001 agtcccagac tttgagccaa ggcaagatgc aaagattgtg actgatgaga aagctaccac
     3061 tttaaccact gcttcggtgg acgacgctgc agtcatcgac gatctcattg ctaagatcga
     3121 ccagtgtagg cataacttgt cgccagactt caggatgaaa cctattcagt tcgaaaagga
     3181 tgatgataca aactatcaca tggatgtgat agcaggacta gccaacatga gggcgagaaa
     3241 ttacagcatc cctgaagtgg acaagctgaa agcaaagttc atagcaggga gaatcatccc
     3301 agccattgcg acttcaacag ccatggctac tgggctggtc tgcctcgagc tgtacaaggt
     3361 ccttgatgga ggacataaag tggaagctta cagaaacaca tttgccaacc tggcacttcc
     3421 actattctcc atggctgaac cacttccacc aaaggtggtg aagcaccgtg acatggcttg
     3481 gacagtttgg gacagatggg ttctcaaggg aaacccaact cttcgtgagg tgttgcagtg
     3541 gctggaggac aaagggctta gcgcatacag catctcttgt ggaagctgtc tactgttcaa
     3601 cagtatgttc acaaggcaca aggagaggat ggacaagaag gttgtggatc tcgctcggga
     3661 tgttgcgaaa gtcgagttac cgccttatcg caaccatctt gatgtagtgg tggcttgtga
     3721 ggatgaagat gacaatgacg tcgatattcc tctcgtctct atctacttca ggtgagctta
     3781 ctattaactt taagaaggag atataccatg tacccatacg atgttcctga ctatgcgggc
     3841 tatccctatg acgtcccgga ctatgcagga ttgtatccat atgacgttcc agattacgcc
     3901 actagagctg cttacccata cgatgttcct gactatgcgg gctatcccta tgacgtcccg
     3961 gactatgcag gattgtatcc atatgacgtt ccagattacg caatgtcgac gccagcaagg
     4021 aagaggttaa tgagggattt caagaggttg cagcaagacc cacctgcggg tattagtggt
     4081 gctccacagg acaacaacat tatgctctgg aatgctgtca tatttgggcc tgatgacaca
     4141 ccatgggatg gaggtacttt caaactctca ctgcagttct ctgaagatta tcccaataaa
     4201 ccaccaacag ttcggtttgt gtcacggatg tttcatccta atatttatgc agatgggagt
     4261 atctgcttgg acattctaca aaaccagtgg agtccaatct atgatgttgc tgctatactt
     4321 acctccatcc agtccttgct ctgtgaccct aatccgaatt ctcctgcaaa ctcggaagct
     4381 gctcggatgt acagcgaaag caagcgcgag tacaacagga gagtgcgtga tgttgttgag
     4441 caaagctgga ctgctgacta ggcttttacg cttttaagcc agccccgaca cccgccaaca
     4501 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4561 accgggatga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     4621 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     4681 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
     4741 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4801 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4861 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4921 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4981 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5041 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5101 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5161 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5221 ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg
     5281 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     5341 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     5401 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     5461 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     5521 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     5581 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagtcga
     5641 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     5701 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     5761 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     5821 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     5881 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     5941 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     6001 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt
     6061 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     6121 attgttcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6181 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6241 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6301 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6361 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6421 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6481 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6541 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6601 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6661 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6721 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6781 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6841 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6901 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6961 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     7021 ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     7081 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     7141 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgact
     7201 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     7261 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     7321 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     7381 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     7441 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     7501 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     7561 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     7621 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     7681 acttccgcgt ttccagactt tacgaaacac ggaaacccaa gaccattcat gttgttgctc
     7741 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     7801 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     7861 tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
     7921 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
     7981 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
     8041 gcgctgccgg cacctgtcct acgagttgca tgataaagaa cacagtcata agtgcggcga
     8101 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     8161 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     8221 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8281 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgacacg
     8341 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     8401 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     8461 gagctgtcct cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     8521 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     8581 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     8641 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     8701 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     8761 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcctc atgggagaaa
     8821 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     8881 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     8941 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     9001 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     9061 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     9121 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     9181 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     9241 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     9301 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     9361 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     9421 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     9481 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaa
//
