LOCUS       sequence-220625-        3694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used for in situ hybridisation or other purposes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Hall TE, Martel N, Lo HP, Xiong Z, Parton RG
  TITLE     A plasmid library of full-length zebrafish rab proteins for in vivo 
            cell biology.
  JOURNAL   Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 
            10.1080/21592799.2017.1301151. eCollection 2017.
  PUBMED    28396820
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Logist."; date: "2017-03-1"; volume: "7(1):e1301151. doi"; pages: " 
            10.1080/21592799.2017.1301151. eCollection 2017"
COMMENT     Sequence Label: Rab20 cds in pGem-T Easy
FEATURES             Location/Qualifiers
     source          1..3694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      75..84
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(118..137)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(119..137)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(144..161)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(144..160)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(153..175)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      301..756
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(388..407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     598..619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        834..938
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(939..1007,1008..1799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1157..1176)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1970..2558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2459..2478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2712..2729
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2846..2867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2882..2887,2888..2905,2906..2912)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2920..2936
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2925..2947
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2944..2960
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2944..2960
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2978..2996
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2978..2995
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ctgtagatct ggagacgcca ctagaggtga gaaggactaa agctggctgc tgtgcataga
       61 atcgaattcc cgcggccgcc atggcggccg ggagcatgcg acgtcgggcc caattcgccc
      121 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      181 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      241 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      301 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      361 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      421 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      481 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      541 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      601 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      661 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      721 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
      781 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
      841 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      901 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      961 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1021 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1081 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1141 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1201 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1261 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1321 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1381 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1441 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1501 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1561 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1621 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1681 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1741 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1801 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1861 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1921 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1981 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2041 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2101 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2161 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2221 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2281 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2341 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2401 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2461 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2521 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2581 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2641 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2701 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2761 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2821 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2881 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2941 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
     3001 tcaagctatg catccaacgc gttgggagct ctcccatatg gtcgacctgc aggcggccgc
     3061 gaattcacta gtgattggga catgaacgtt ggaaagactt ctctcctcca cagatacacg
     3121 gagaggaagt ttaaggacac gataagcacc gtcggagggg cgtttttcct caaacagtgg
     3181 ggtccctaca acatttccat atgggatact gcaggtcgag agcagtttca cggtctgggc
     3241 tccatgtact gccgtggcgc agctgctgtc atcctaacat atgatgtcac caactggcag
     3301 agcctggcgg agctggagaa ccgcttcctg tccttgactg acactgccaa cgcagactgc
     3361 atcttcgccc ttgtggggaa caaagcggac ctgaccgacc cgttcgctca agagcctgac
     3421 agcgagagtg ggagcgaccc gcagcctgta gcctcctgcc cgactcctcc tgccagccct
     3481 gtccagcaca aacaggtgag cagagaggac gctgtggctc tgtacgcccg cgttctccga
     3541 tacaaaggcc tggacgagaa gacctgcctg cccgccgaga ggatgtgctt cgagaccagc
     3601 gctaaaacag gttacaacgt ggacgctttg ttcgagacct tatttgattt agtactgcca
     3661 tccattctga agaagaggag cgagggtgaa gcca
//
