LOCUS       sequence-220635-       12323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of the TFDP2 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Rady B, Chen Y, Vaca P, Wang Q, Wang Y, Salmon P, Oberholzer J
  TITLE     Overexpression of E2F3 promotes proliferation of functional human 
            beta cells without induction of apoptosis.
  JOURNAL   Cell Cycle. 2013 Aug 15;12(16):2691-702. doi: 10.4161/cc.25834. Epub
            2013 Jul 29.
  PUBMED    23907129
REFERENCE   2  (bases 1 to 12323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/cc.25834"; 
            journalName: "Cell Cycle"; date: "2013-08-15- 15"; volume: "12"; 
            issue: "16"; pages: "2691-702"
COMMENT     Sequence Label: pWPI-TFDP2
FEATURES             Location/Qualifiers
     source          1..12323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    48..165
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    197..221
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1386..1410)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1434..2001
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1452..2003
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1596..1613)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1823..1842
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2006..2008,2009..2011,2012..2722)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2006..2008,2009..2011,2012..2722)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2051..2072)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2312..2331)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2659..2680
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2801..2817)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2802..2818)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    2831..3419
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2884..2904)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3302..3313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3563..3596)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             3616..3796
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3821..3839)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3822..3839)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4129..4148)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4362..4381)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4481..4503
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4541..4559)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4627..4731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4732..4800,4801..5592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4950..4969)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5763..6351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6252..6271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6505..6522
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6592..6612)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6597..6926
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6777..6912
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8060..8125
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8255..8275
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8700..8834
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8737..8756)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8791..8810
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9002..9635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9682..9807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10300..10533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10718..10762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11145..12323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          11376..12314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     12271..12291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggaatttcga catttaaatt taattaatct cgacggtatc ggttaacttt taaaagaaaa
       61 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      121 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga tcacgagact
      181 agcctcgagg tttatcacaa gtttgtacaa aaaagcaggc accatgatta taagcacacc
      241 acagagacta accagttcag gaagtgttct gattgggagt ccatataccc ctgcaccagc
      301 aatggttact cagacacaca tagcagaagc tactggctgg gtccctggtg atagaaaacg
      361 ggctagaaaa tttatagact ctgatttttc agaaagtaaa cgaagcaaaa aaggagataa
      421 aaatgggaaa ggcttgagac acttttcaat gaaagtgtgt gagaaagttc aacgaaaagg
      481 tacaacatcg tacaatgaag tcgctgatga gctggtgtca gagttcacca attcaaataa
      541 ccatttggct gctgattcgc aggcttatga tcagaagaac attaggcgaa gagtttatga
      601 tgctttaaat gtgctaatgg caatgaacat aatttcaaag gaaaaaaaag aaatcaagtg
      661 gattggcctg cctaccaatt ctgctcagga atgtcagaat ctggagatag agaagcagag
      721 gcggatagaa cggataaagc agaagcgggc ccagctgcaa gaacttctcc tacagcaaat
      781 cgctttcaaa aacctggtac agagaaatcg acaaaatgag cagcaaaacc agggcccgcc
      841 ggctctgaac tctaccattc agctgccatt cataatcatc aatacaagca gaaaaacagt
      901 catagattgc agcatctcca gtgacaagtt tgagtatctt ttcaattttg acaacacctt
      961 tgagatccat gatgacatag aagtactaaa gcggatggga atgtcgtttg gcctggagtc
     1021 aggcaaatgc tctctggagg atctgaaact tgcgaaatcc ctggtgccaa aggctttaga
     1081 aggttatatc acagatatct ccacaggacc ttcttggtta aatcagggac tacttctgaa
     1141 ctctacccaa tcagtttcaa atttagacct gaccactggt gccaccttac cccagtcaag
     1201 tgtaaaccaa gggttatgct tggatgcaga agtggcctta gcaactgggc agttcctggc
     1261 cccaaacagt caccagtcca gcagtgcggc ctctcactgc tccgagtccc gaggcgagac
     1321 cccctgttcg ttcaatgatg aagatgagga agatgatgag gaggattcct cctccccaga
     1381 atagaaccca gctttcttgt acaaagtggt gataaactac gggctgcagg aattccgccc
     1441 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     1501 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     1561 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     1621 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     1681 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     1741 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     1801 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     1861 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     1921 atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     1981 ctttgaaaaa cacgatgata ataccatggt gagcaagggc gaggagctgt tcaccggggt
     2041 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2101 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     2161 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     2221 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2281 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2341 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2401 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2461 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     2521 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     2581 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     2641 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     2701 cggcatggac gagctgtaca agtccggact cagatctcga ctagctagta gctagctagc
     2761 tagtcgagct caagcttcga attcgatatc aagcttatcg cgataccgtc gacctcgagg
     2821 gaattccgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     2881 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     2941 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3001 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3061 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3121 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3181 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     3241 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     3301 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     3361 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     3421 tcgggaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     3481 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3541 atgggatcaa ttcaccatgg gaataacttc gtatagcata cattatacga agttatgctg
     3601 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3661 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     3721 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     3781 tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc
     3841 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
     3901 acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa
     3961 ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc
     4021 acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg
     4081 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     4141 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     4201 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     4261 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
     4321 aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc
     4381 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4441 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4501 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4561 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4621 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4681 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4741 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4801 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4861 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4921 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4981 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5041 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5101 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5161 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5221 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5281 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5341 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5401 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5461 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5521 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5581 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5641 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5701 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5761 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5821 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5881 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5941 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6001 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6061 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6121 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6181 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6241 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6301 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6361 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6421 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6481 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6541 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt
     6601 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6661 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     6721 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     6781 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6841 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     6901 tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt
     6961 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
     7021 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
     7081 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
     7141 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
     7201 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac
     7261 tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg
     7321 acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg
     7381 ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa
     7441 aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg
     7501 ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg
     7561 tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt
     7621 ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca
     7681 caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg
     7741 ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa
     7801 accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac
     7861 ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac
     7921 gatgatttat acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc
     7981 ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     8041 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     8101 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     8161 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     8221 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     8281 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     8341 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     8401 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     8461 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     8521 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     8581 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     8641 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     8701 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8761 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8821 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
     8881 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
     8941 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
     9001 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
     9061 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     9121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     9181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     9241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     9301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     9361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     9421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     9481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     9661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     9721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     9781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     9841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     9901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     9961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    10021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    10081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    10141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
    10201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
    10261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
    10321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
    10381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    10441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
    10501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
    10561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    10621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    10681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    10741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    10801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    10861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    10921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
    10981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
    11041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
    11101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
    11161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
    11221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
    11281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
    11341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
    11401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
    11461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
    11521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
    11581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
    11641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
    11701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
    11761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
    11821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
    11881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
    11941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
    12001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
    12061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
    12121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
    12181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
    12241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
    12301 ttcttccatt tcaggtgtcg tga
//
