LOCUS       sequence-220639-        8122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, 
            Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau 
            K, Schnoegl S, Garner CC, Wanker EE
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology 
            for quantitative mapping of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Mol Syst Biol. 2018 Jul 11;14(7):e8071. doi: 10.15252/msb.20178071.
  PUBMED    29997244
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2018-07-11"; volume: "14(7):e8071. doi"; pages: " 
            10.15252/msb.20178071"
COMMENT     Sequence Label: pcDNA3.1 PA-mCit-GW
FEATURES             Location/Qualifiers
     source          1..8122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        146..525
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        526..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     774..793
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        774..792
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             818..991
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             992..1165
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             1507..1518
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    1889..2013
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2038..2043,2044..2061,2062..2068)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2122..2802
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(2188..2207)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3122..3427
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3306..3325
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3468..3592)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3634..3651)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3640..3751
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3900..4328
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3987..4006)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4197..4218
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4337..4357)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4342..4671
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4522..4657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4584..4603
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4738..5532
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4792..4811)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5402..5421
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5706..5827
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5743..5762)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5797..5816
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5876..5892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5876..5892)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5889..5911)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5900..5916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5924..5930,5931..5948,5949..5954))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5969..5990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6107..6124)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6278..6866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6358..6377)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7037..7828,7829..7897))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7660..7679
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7898..8002)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8077..8096)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
       61 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
      121 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
      781 actcactata gggagaccca agctggctag caccatggtg gacaacaaat tcaacaaaga
      841 acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag aacaacgaaa
      901 cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt tagcagaagc
      961 taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca aagaacaaca
     1021 aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac gaaacgcctt
     1081 catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag aagctaaaaa
     1141 gctaaatggt gctcaggcgc cgaaagtaga cgcgaactcg gaattcggct cgggctcggt
     1201 atcgaaaggt gaagagctgt ttactggcgt cgtgcccatt ttggtggaac ttgacggtga
     1261 cgtgaacggt cacaaattct cagtctcggg agaaggcgag ggggacgcga catatgggaa
     1321 actcacactg aagttcatct gcacgactgg aaagctgcct gtcccttggc ctacactcgt
     1381 cacgacgttc ggttatggtt tgatgtgttt cgcaagatat cctgaccaca tgaaacagca
     1441 tgatttcttt aagagcgcca tgcccgaagg ttatgtccag gagaggacta tcttctttaa
     1501 ggacgatggg aactacaaga ctcgggcaga ggtgaaattt gagggggaca cgcttgtgaa
     1561 tagaatcgaa ctcaaaggca tcgatttcaa ggaggatgga aacattctcg gacacaaact
     1621 tgagtacaat tacaacagcc acaacgtgta tatcatggcg gacaaacaga agaatggaat
     1681 caaagtgaac ttcaagatca gacacaatat cgaagatggg tcagtgcagc tggctgatca
     1741 ttaccagcag aatacgccca tcggggacgg tccagtcttg ttgccggata accactacct
     1801 ttcataccag tcgaagttgt ccaaagaccc caacgagaag agggatcaca tggtgcttct
     1861 tgaatttgtc acagccgctg gtaagcttac aagtttgtac aaaaaagctg aacgagaaac
     1921 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
     1981 atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt
     2041 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt
     2101 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     2161 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     2221 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     2281 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     2341 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     2401 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     2461 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     2521 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     2581 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     2641 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     2701 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     2761 gagtggcagg cggggcgtaa tctagaggat ccggcttact aaaagccaga taacagtatg
     2821 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
     2881 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
     2941 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
     3001 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
     3061 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga
     3121 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     3181 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg
     3241 tctgctgtca gataaagtcc cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     3301 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
     3361 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     3421 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
     3481 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     3541 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgtaactcg
     3601 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3661 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3721 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3781 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggcaggcatg ctggggatgc
     3841 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     3901 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3961 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4021 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4081 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4141 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4201 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4261 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4321 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4381 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4441 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4501 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4561 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4621 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     4681 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     4741 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4801 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4861 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     4921 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4981 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5041 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5101 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5161 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5221 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5281 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5341 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5401 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5461 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5521 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5581 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5641 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5701 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5761 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5821 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     5881 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5941 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6001 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6061 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6121 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6181 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6241 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6301 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6361 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6421 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6481 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6541 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6601 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6661 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6721 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6781 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6841 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6901 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6961 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7021 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7081 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7141 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7201 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7261 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7321 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7381 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7441 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7501 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7561 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7621 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7681 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7741 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7801 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7861 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7921 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7981 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     8041 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     8101 gttaagccag tatctgctcc ct
//
