LOCUS       sequence-220718-       10567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector encoding BFP-BSD-Nkx2-5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10567)
  AUTHORS   Yu JSL, Palano G, Lim C, Moggio A, Drowley L, Plowright AT, 
            Bohlooly-Y M, Rosen BS, Hansson EM, Wang QD, Yusa K
  TITLE     CRISPR-KO screen identifies Dmap1 as a regulator of 
            chemically-induced reprogramming and differentiation of cardiac 
            progenitors.
  JOURNAL   Stem Cells. 2019 Apr 1. doi: 10.1002/stem.3012.
  PUBMED    30932271
REFERENCE   2  (bases 1 to 10567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2019 Apr 1. doi: 10.1002/stem.3012."
COMMENT     Sequence Label: pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5
FEATURES             Location/Qualifiers
     source          1..10567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        960..1158
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1113..1133
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1176..1356
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1403..1528
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2027..2260
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2445..2489
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2787..2904
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2979..3227
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2979..2999
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3150..3169
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3761..4946
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3992..4938
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4895..4915
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4960..4969
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4966..5667
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     CDS             5686..5739
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5746..6141
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             6160..6213
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(7242..7262)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7660..7671)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8123..8140)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8129..8353
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8379..8399)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8379..8397)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8418..8434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8418..8434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8431..8453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8442..8458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8466..8472,8473..8490,8491..8496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8511..8532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8649..8666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8820..9408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8900..8919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9579..10370,10371..10439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10202..10221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10440..10544)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgactagtac
      661 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      721 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      781 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      841 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      901 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      961 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     1021 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     1081 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     1141 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     1201 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1261 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1321 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     1381 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     1441 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1501 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1561 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1621 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1681 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1741 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1801 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1861 gcaagcggcc ggccgcgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1921 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1981 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2041 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2101 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2161 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2221 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2281 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2341 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2401 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2461 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2521 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2581 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2641 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2701 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     2761 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2821 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2881 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     2941 ttagtaccgg gccctacgcg tccaaggtcg ggcaggaaga gggcctattt cccatgattc
     3001 cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg
     3061 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     3121 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     3181 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgga gacgcatgac
     3241 tcgtctcagt ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg
     3301 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tacgcgtgag gggcgcggaa
     3361 gccggggcgg aggaggagcc aaccccgggc tgggctgaga cccgcagagg aagacgctct
     3421 agggatttgt cccggactag cgagatggca aggctgagga cgggaggctg attgagaggc
     3481 gaaggtacac cctaatctca atacaacctt tggagctaag ccagcaatgg tagagggaag
     3541 attctgcacg tcccttccag gcggcctccc cgtcaccacc ccccccaacc cgccccgacc
     3601 ggagctgaga gtaattcata caaaaggact cgcccctgcc ttggggaatc ccagggaccg
     3661 tcgttaaact cccactaacg tagaacccag agatcgctgc gttcccgccc cctcacccgc
     3721 ccgctctcgt catcactgag gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg
     3781 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     3841 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     3901 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     3961 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     4021 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacgc ccctggctgc
     4081 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     4141 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcttggg cgctggggcc
     4201 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     4261 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     4321 atgcgggcca agatcgatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     4381 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     4441 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     4501 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     4561 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     4621 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     4681 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     4741 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     4801 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     4861 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     4921 ttttttcttc catttcaggt gtcgtgggaa gcttggcgcg ccaccatggg cagcgagctg
     4981 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
     5041 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     5101 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
     5161 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
     5221 cctgagggct tcacatggga gagagtcacc acatacgagg acgggggcgt gctgaccgct
     5281 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     5341 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
     5401 gagaccctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
     5461 gtgggcggga gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct
     5521 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
     5581 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
     5641 cctagcaaac tggggcacaa gcttaatgga gggggcagcg gcggggaagg aaggggctcc
     5701 ctgctcacct gcggcgacgt ggaagagaac cccggacctg tcgacatggc caagcctttg
     5761 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     5821 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     5881 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     5941 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     6001 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     6061 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     6121 tctggttatg tgtgggaggg cggagggggc agcggcgggg aaggaagggg ctccctgctc
     6181 acctgcggcg acgtggaaga gaaccccgga cctatgcata tgttccccag ccctgcgctc
     6241 acacccacgc ctttctcagt caaagacatc ctgaacctgg agcagcagca gcgtagcctg
     6301 gcgtctgggg acctgtctgc gcgcctcgag gccaccctgg cccctgcctc ctgcatgctg
     6361 gccgccttca agcccgaggc ctactctggc cccgaggcgg cagcgtccgg cctggcagag
     6421 ctgcgcgcgg agatgggccc cgcgccttcg ccccccaagt gctctcctgc tttcccagcc
     6481 gcccccacat tttacccggg agcctacggt gaccctgacc cagccaaaga ccctcgggcg
     6541 gataaaaaag agctgtgcgc gctgcagaag gcagtggagc tggacaaagc cgagacggat
     6601 ggcgccgaga gaccacgcgc acggcggcga cggaagccac gcgtgctctt ctcgcaggcg
     6661 caggtctacg agctggagcg gcgcttcaag caacagcggt acctgtcggc gccagagcgc
     6721 gaccagctgg ccagcgtgct gaagctcacg tccacgcagg tcaagatctg gttccagaac
     6781 cgtcgctaca agtgcaagcg acagcggcag gaccagactc tggagcttct ggggccgccg
     6841 ccgccgcccg cgcgcaggat cgcggtgccc gtgctggtgc gcgacgggaa gccctgcctg
     6901 ggggaccccg cggcctacgc tcccgcctac ggcgtgggtc tcaatgccta tggctacaac
     6961 gcctacccct accccagcta cggcggcgcg gcctgcagtc ccggctacag ctgcgccgcc
     7021 taccccgctg cgccccccgc cgcgcagccc cccgccgcct ccgccaacag caacttcgtg
     7081 aactttggcg tcggggactt gaacaccgtg cagagtcccg ggatgccgca gggcaattcg
     7141 ggcgtctcca cgctgcacgg catccgagcc tggtaggcta gcgcggccgc tcaacctctg
     7201 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7261 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7321 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7381 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7441 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7501 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7561 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7621 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7681 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7741 cagacgagtc ggatctccct ttgggccgcc tccccgcagg tacctttaag accaatgact
     7801 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7861 attcactccc aaagaagtca agatctgctt tttgcctgta ctgggtctct ctggttagac
     7921 cagagtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7981 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8041 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8101 gcagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     8161 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     8221 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8281 gtggggcagg acagcaaggg ggaggattgg gaagtcaata gcaggcatgc tggggatgcg
     8341 gtgggctcta tgggcggccg ttaattaagc ttttgttccc tttagtgagg gttaattgcg
     8401 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8461 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8521 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8581 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8641 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8701 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8761 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8821 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8881 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8941 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     9001 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9061 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9121 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9181 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9241 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9301 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9361 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9421 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9481 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9661 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9721 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9781 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9841 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9901 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9961 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    10021 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10081 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10141 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10201 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10261 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10321 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10381 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10441 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10501 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10561 gtgccac
//
