LOCUS       sequence-22073-e         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Feinstein P, Bozza T, Rodriguez I, Vassalli A, Mombaerts P
  TITLE     Axon guidance of mouse olfactory sensory neurons by odorant 
            receptors and the beta2 adrenergic receptor.
  JOURNAL   Cell. 2004 Jun 11. 117(6):833-46.
  PUBMED    15186782
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2004 
            Jun 11. 117(6):833-46."
COMMENT     Sequence Label: GFP->M72-IRES-tauLacZ LNL TV
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(162..164,165..167,168..680)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKICHNIE"
     primer_bind     complement(207..228)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(468..487)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agatgcatct atcataactg ggggtagctc agcaagtgcc agatgcagaa aaccagctgc
       61 ttcaagcaga acaagaacaa ccattcacaa actccttctc tcccacccca caggcttccc
      121 acccagatac atgtttaatt aacgatccac cggtcgccac catggtgagc aagggcgagg
      181 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      241 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      301 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      361 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      421 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      481 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      541 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      601 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      661 agatctgcca caacatcgag gacggcagcg tgcagctcgc cg
//
