LOCUS       sequence-220756-         449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of gRNA targeting COSMC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Narimatsu Y, Joshi HJ, Zhang Y, Gomes C, Chen YH, Lorenzetti F, 
            Furukawa S, Schjoldager K, Hansen L, Clausen H, Bennett EP, Wandall 
            HH
  TITLE     A validated gRNA library for CRISPR/Cas9 targeting of the human 
            glycosyltransferase genome.
  JOURNAL   Glycobiology. 2018 Jan 5. pii: 4791732. doi: 10.1093/glycob/cwx101.
  PUBMED    29315387
REFERENCE   2  (bases 1 to 449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Glycobiology. 2018 Jan 5. pii: 4791732. doi: 
            10.1093/glycob/cwx101."
COMMENT     Sequence Label: gh22
FEATURES             Location/Qualifiers
     source          1..449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     104..123
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        202..277
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
       61 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
      121 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
      181 cgtaggtgat gatgctcatg ggttttagag ctagaaatag caagttaaaa taaggctagt
      241 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgttttag agctagaaat
      301 agcaagttaa aataaggcta gtccgttttt agcgcgtgcg ccaattctgc agacaaatgg
      361 ctctagaggg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
      421 ctcgctcact gaggccgggc gaccaaagg
//
