LOCUS       sequence-220757-         701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of gRNA targeting POMT1, Cas9, and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Narimatsu Y, Joshi HJ, Zhang Y, Gomes C, Chen YH, Lorenzetti F, 
            Furukawa S, Schjoldager K, Hansen L, Clausen H, Bennett EP, Wandall 
            HH
  TITLE     A validated gRNA library for CRISPR/Cas9 targeting of the human 
            glycosyltransferase genome.
  JOURNAL   Glycobiology. 2018 Jan 5. pii: 4791732. doi: 10.1093/glycob/cwx101.
  PUBMED    29315387
REFERENCE   2  (bases 1 to 701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Glycobiology. 2018 Jan 5. pii: 4791732. doi: 
            10.1093/glycob/cwx101."
COMMENT     Sequence Label: gh23
FEATURES             Location/Qualifiers
     source          1..701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        162..237
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        334..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
       61 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      121 ttgtggaaag gacgaaacac cgagctccaa cactatctgg tgttttagag ctagaaatag
      181 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
      241 tttgttttag agctagaaat agcaagttaa aataaggcta gtccgttttt agcgcgtgcg
      301 ccaattctgc agacaaatgg ctctagaggt acccgttaca taacttacgg taaatggccc
      361 gcctggctga ccgcccaacg acccccgccc attgacgtca atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattgtgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
      661 ccccccctcc ccacccccaa ttttgtattt atttattttt t
//
