LOCUS       sequence-220864-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  single guide RNA targeting Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Hong AL, Tseng YY, Wala JA, Kim WJ, Kynnap BD, Doshi MB, Kugener G, 
            Sandoval GJ, Howard TP, Li J, Yang X, Tillgren M, Ghandi M, Sayeed 
            A, Deasy R, Ward A, McSteen B, Labella KM, Keskula P, Tracy A, 
            Connor C, Clinton CM, Church AJ, Crompton BD, Janeway KA, Van Hare 
            B, Sandak D, Gjoerup O, Bandopadhayay P, Clemons PA, Schreiber SL, 
            Root DE, Gokhale PC, Chi SN, Mullen EA, Roberts CW, Kadoch C, 
            Beroukhim R, Ligon KL, Boehm JS, Hahn WC
  TITLE     Renal medullary carcinomas depend upon SMARCB1 loss and are 
            sensitive to proteasome inhibition.
  JOURNAL   Elife. 2019 Mar 12;8. pii: 44161. doi: 10.7554/eLife.44161.
  PUBMED    30860482
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-12"; volume: "8. pii: 44161. doi"; pages: " 
            10.7554/eLife.44161"
COMMENT     Sequence Label: pXPR_BRD003 Luciferase
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        63..138
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    194..311
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        469..680
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 gatttcttgg ctttatatat cttgtggaag gacgaaacac cgaattgtct tgtccctatc
       61 gagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      121 gtggcaccga gtcggtgctt ttttaagctt ggcgtaacta gatcttgaga caaatggcag
      181 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
      241 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
      301 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
      361 gggcccgggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
      421 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
      481 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga
      541 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      601 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
      661 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
      721 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
      781 atcccgagct tcgggttgga
//
