LOCUS       sequence-220865-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  single guide RNA targeting PSMB5; guide 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Hong AL, Tseng YY, Wala JA, Kim WJ, Kynnap BD, Doshi MB, Kugener G, 
            Sandoval GJ, Howard TP, Li J, Yang X, Tillgren M, Ghandi M, Sayeed 
            A, Deasy R, Ward A, McSteen B, Labella KM, Keskula P, Tracy A, 
            Connor C, Clinton CM, Church AJ, Crompton BD, Janeway KA, Van Hare 
            B, Sandak D, Gjoerup O, Bandopadhayay P, Clemons PA, Schreiber SL, 
            Root DE, Gokhale PC, Chi SN, Mullen EA, Roberts CW, Kadoch C, 
            Beroukhim R, Ligon KL, Boehm JS, Hahn WC
  TITLE     Renal medullary carcinomas depend upon SMARCB1 loss and are 
            sensitive to proteasome inhibition.
  JOURNAL   Elife. 2019 Mar 12;8. pii: 44161. doi: 10.7554/eLife.44161.
  PUBMED    30860482
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-12"; volume: "8. pii: 44161. doi"; pages: " 
            10.7554/eLife.44161"
COMMENT     Sequence Label: pXPR_BRD003 PSMB5 guide 1
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        66..141
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    197..314
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        472..683
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ttcgatttct tggctttata tatcttgtgg aaggacgaaa caccgtctac tacgtggaca
       61 gtgaagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
      121 aaagtggcac cgagtcggtg cttttttaag cttggcgtaa ctagatcttg agacaaatgg
      181 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      241 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      301 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
      361 agggggcccg ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
      421 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
      481 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct
      541 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
      601 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
      661 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
      721 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
      781 ttgatcccga gcttcgggtt
//
