LOCUS       sequence-220867-       11115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination vector expressing plant-codon-optimized Cas9 under 35S 
            promoter, with sgRNAs be shuffled in; seed coat specific red 
            fluorescence for screening trangene free.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Wu R, Lucke M, Jang YT, Zhu W, Symeonidi E, Wang C, Fitz J, Xi W, 
            Schwab R, Weigel D
  TITLE     An efficient CRISPR vector toolbox for engineering large deletions 
            in Arabidopsis thaliana.
  JOURNAL   Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. 
            eCollection 2018.
  PUBMED    30083222
REFERENCE   2  (bases 1 to 11115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2018-08-2"; volume: "14:65. doi"; pages: " 
            10.1186/s13007-018-0330-7. eCollection 2018"
COMMENT     Sequence Label: pRW006-SM-destination-pro35S-Cas9
FEATURES             Location/Qualifiers
     source          1..11115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        1086..1431
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1374..1394
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1460..1507
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             1514..1534
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1559..2995,3185..5848)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          2996..3184
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             5849..5896
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5902..6543
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(6766..7089)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPCVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     6999..7015
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7085..7101)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7085..7101)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7098..7120)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7109..7125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7133..7139,7140..7157,7158..7163))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7178..7199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7316..7333)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(7712..7964)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(8248..8267)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8517..8535
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    9134..9158
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9249..9837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9329..9348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10008..10796)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(11087..11115,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagcatt cggtccccag
      601 attagccttt tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca
      661 gcacgtctca tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc
      721 aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa
      781 gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcac
      841 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac tgaatcaaag
      901 gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa
      961 cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg
     1021 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg
     1081 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
     1141 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
     1201 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
     1261 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     1321 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
     1381 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggacg
     1441 agctaacagc ggatccatgg attacaagga tgatgatgat aaggattaca aggatgatga
     1501 tgataagatg gctccaaaga agaagagaaa ggttggaatc cacggagttc cagctgctga
     1561 taagaagtac tctatcggac ttgacatcgg aaccaactct gttggatggg ctgttatcac
     1621 cgatgagtac aaggttccat ctaagaagtt caaggttctt ggaaacaccg atagacactc
     1681 tatcaagaag aaccttatcg gtgctcttct tttcgattct ggagaaaccg ctgaggctac
     1741 cagattgaag agaaccgcta gaagaagata caccagaaga aagaacagaa tctgctacct
     1801 tcaggaaatc ttctctaacg agatggctaa ggttgatgat tctttcttcc acagacttga
     1861 ggagtctttc cttgttgagg aggataagaa gcacgagaga cacccaatct tcggaaacat
     1921 cgttgatgag gttgcttacc acgagaagta cccaaccatc taccacctta gaaagaagtt
     1981 ggttgattct accgataagg ctgatcttag acttatctac cttgctcttg ctcacatgat
     2041 caagttcaga ggacacttcc ttatcgaggg tgaccttaac ccagataact ctgatgttga
     2101 taagttgttc atccagcttg ttcagaccta caaccagctt ttcgaggaga acccaatcaa
     2161 cgcttctgga gttgatgcta aggctatcct ttctgctaga ctttctaagt ctcgtagact
     2221 tgagaacctt atcgctcagc ttccaggaga gaagaagaac ggacttttcg gaaaccttat
     2281 cgctctttct cttggactta ccccaaactt caagtctaac ttcgatcttg ctgaggatgc
     2341 taagttgcag ctttctaagg atacctacga tgatgatctt gataaccttc ttgctcagat
     2401 cggagatcag tacgctgatc ttttccttgc tgctaagaac ctttctgatg ctatccttct
     2461 ttctgacatc cttagagtta acaccgagat caccaaggct ccactttctg cttctatgat
     2521 caagagatac gatgagcacc accaggatct tacccttttg aaggctcttg ttagacagca
     2581 gcttccagag aagtacaagg aaatcttctt cgatcagtct aagaacggat acgctggata
     2641 catcgatgga ggagcttctc aggaggagtt ctacaagttc atcaagccaa tccttgagaa
     2701 gatggatgga accgaggagc ttcttgttaa gttgaacaga gaggatcttc ttagaaagca
     2761 gagaaccttc gataacggat ctatcccaca ccagatccac cttggagagc ttcacgctat
     2821 ccttcgtaga caggaggatt tctacccatt cttgaaggat aacagagaga agatcgagaa
     2881 gatccttacc ttcagaatcc catactacgt tggaccactt gctagaggaa actctcgttt
     2941 cgcttggatg accagaaagt ctgaggaaac catcacccct tggaacttcg aggaggtaag
     3001 tttctgcttc tacctttgat atatatataa taattatcat taattagtag taatataata
     3061 tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt
     3121 tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt
     3181 gcaggttgtt gataagggag cttctgctca gtctttcatc gagagaatga ccaacttcga
     3241 taagaacctt ccaaacgaga aggttcttcc aaagcactct cttctttacg agtacttcac
     3301 cgtttacaac gagcttacca aggttaagta cgttaccgag ggaatgagaa agccagcttt
     3361 cctttctgga gagcagaaga aggctatcgt tgatcttctt ttcaagacca acagaaaggt
     3421 taccgttaag cagttgaagg aggattactt caagaagatc gagtgcttcg attctgttga
     3481 aatctctgga gttgaggata gattcaacgc ttctcttgga acctaccacg atcttttgaa
     3541 gatcatcaag gataaggatt tccttgataa cgaggagaac gaggacatcc ttgaggacat
     3601 cgttcttacc cttacccttt tcgaggatag agagatgatc gaggagagac tcaagaccta
     3661 cgctcacctt ttcgatgata aggttatgaa gcagttgaag agaagaagat acaccggatg
     3721 gggtagactt tctcgtaagt tgatcaacgg aatcagagat aagcagtctg gaaagaccat
     3781 ccttgatttc ttgaagtctg atggattcgc taacagaaac ttcatgcagc ttatccacga
     3841 tgattctctt accttcaagg aggacatcca gaaggctcag gtttctggac agggagattc
     3901 tcttcacgag cacatcgcta accttgctgg atctccagct atcaagaagg gaatccttca
     3961 gaccgttaag gttgttgatg agcttgttaa ggttatgggt agacacaagc cagagaacat
     4021 cgttatcgag atggctagag agaaccagac cacccagaag ggacagaaga actctcgtga
     4081 gagaatgaag agaatcgagg agggaatcaa ggagcttgga tctcaaatct tgaaggagca
     4141 cccagttgag aacacccagc ttcagaacga gaagttgtac ctttactacc ttcagaacgg
     4201 aagagatatg tacgttgatc aggagcttga catcaacaga ctttctgatt acgatgttga
     4261 tcacatcgtt ccacagtctt tcttgaagga tgattctatc gataacaagg ttcttacccg
     4321 ttctgataag aacagaggaa agtctgataa cgttccatct gaggaggttg ttaagaagat
     4381 gaagaactac tggagacagc ttcttaacgc taagttgatc acccagagaa agttcgataa
     4441 ccttaccaag gctgagagag gaggactttc tgagcttgat aaggctggat tcatcaagag
     4501 acagcttgtt gaaaccagac agatcaccaa gcacgttgct cagatccttg attctcgtat
     4561 gaacaccaag tacgatgaga acgataagtt gatcagagag gttaaggtta tcaccttgaa
     4621 gtctaagttg gtttctgatt tcagaaagga tttccagttc tacaaggtta gagagatcaa
     4681 caactaccac cacgctcacg atgcttacct taacgctgtt gttggaaccg ctcttatcaa
     4741 gaagtaccca aagttggagt ctgagttcgt ttacggagat tacaaggttt acgatgttag
     4801 aaagatgatc gctaagtctg agcaggagat cggaaaggct accgctaagt acttcttcta
     4861 ctctaacatc atgaacttct tcaagaccga gatcaccctt gctaacggag agatcagaaa
     4921 gaggccactt atcgaaacca acggagaaac cggagagatc gtttgggata agggaagaga
     4981 tttcgctacc gttagaaagg ttctttctat gccacaggtt aacatcgtta agaaaaccga
     5041 ggttcagacc ggaggattct ctaaggagtc tatccttcca aagagaaact ctgataagtt
     5101 gatcgctaga aagaaggatt gggacccaaa gaagtacgga ggattcgatt ctccaaccgt
     5161 tgcttactct gttcttgttg ttgctaaggt tgagaaggga aagtctaaga agttgaagtc
     5221 tgttaaggag cttcttggaa tcaccatcat ggagcgttct tctttcgaga agaacccaat
     5281 cgatttcctt gaggctaagg gatacaagga ggttaagaag gatcttatca tcaagttgcc
     5341 aaagtactct cttttcgagc ttgagaacgg aagaaagaga atgcttgctt ctgctggaga
     5401 gcttcagaag ggaaacgagc ttgctcttcc atctaagtac gttaacttcc tttaccttgc
     5461 ttctcactac gagaagttga agggatctcc agaggataac gagcagaagc agcttttcgt
     5521 tgagcagcac aagcactacc ttgatgagat catcgagcaa atctctgagt tctctaagag
     5581 agttatcctt gctgatgcta accttgataa ggttctttct gcttacaaca agcacagaga
     5641 taagccaatc agagagcagg ctgagaacat catccacctt ttcaccctta ccaaccttgg
     5701 tgctccagct gctttcaagt acttcgatac caccatcgat agaaaaagat acacctctac
     5761 caaggaggtt cttgatgcta cccttatcca ccagtctatc accggacttt acgagaccag
     5821 aatcgatctt tctcagcttg gaggagataa gagaccagct gctaccaaga aggctggaca
     5881 ggctaagaag aagaagtgaa aagctttcgt tcgtatcatc ggtttcgaca acgttcgtca
     5941 agttcaatgc atcagtttca ttgcgcacac accagaatcc tactgagttt gagtattatg
     6001 gcattgggaa aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt
     6061 attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat
     6121 atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg
     6181 ttgaatttga aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat
     6241 cgtggcctct aatgaccgaa gttaatatga ggagtaaaac acttgtagtt gtaccattat
     6301 gcttattcac taggcaacaa atatattttc agacctagaa aagctgcaaa tgttactgaa
     6361 tacaagtatg tcctcttgtg ttttagacat ttatgaactt tcctttatgt aattttccag
     6421 aatccttgtc agattctaat cattgcttta taattatagt tatactcatg gatttgtagt
     6481 tgagtatgaa aatatttttt aatgcatttt atgacttgcc aattgattga caacatgcat
     6541 caatcggtac cacctagtag aagagctcag tgagaccgaa ttcgcttact aaaagccaga
     6601 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat
     6661 acccgaagta tgtcaaaaag aggtgtgcta agcttcggat gccgggagca gacaagcccg
     6721 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     6781 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     6841 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     6901 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     6961 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     7021 cgagctcggt acacagggat cttcaagagt cgacctgcag gcatgcaggc ttggcgtaat
     7081 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7141 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7201 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7261 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7321 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcacta gtaggaggta
     7381 atcaatgctg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7441 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7501 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     7561 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
     7621 ctctgatgcc gcatgatcat ctggcggacc tcttcggagg tccgcttttt tttctcgagg
     7681 gtctcaacta gctcttcatg ggtattctag agataattta tttgaaaatt cataagaaaa
     7741 gcaaacgtta catgaattga tgaaacaata caaagacaga taaagccacg cacatttagg
     7801 atattggccg agattactga atattgagta agatcacgga atttctgaca ggagcatgtc
     7861 ttcaattcag cccaaatggc agttgaaata ctcaaaccgc cccatatgca ggagcggatc
     7921 attcattgtt tgtttggttg cctttgccaa catgggagtc caagagctct cacttgtaca
     7981 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
     8041 tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag gcgccgggca
     8101 gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt
     8161 ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc
     8221 gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg tcggagggga
     8281 agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg gaggagtcct
     8341 gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac ttgaagccct
     8401 cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg taggccttgg
     8461 agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg ccacccttgg
     8521 tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc tcgccctcga
     8581 tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc atgaactcct
     8641 tgatgatggc catgttatcc attgttcgta ccgttttgct atttgtgtat gttttcttgt
     8701 tttggtttga gtgaagagtg tagtgaaggg ttggtttgta tttataggtg agaaaggaga
     8761 tttgcatggc aatcacgtgt aagaatgcat gcatgcatga gtgtgtgtgg agagagagag
     8821 atgctcttac gttgttgtcg ggattatgtg ttggaaaaga gtgacaagtg acgaacaaaa
     8881 caataccact catcatagct ccgcttatgc atgctatgtg tgatttgtag ctcttgttct
     8941 gacatgacac tatgaacaat tttttaaaaa gaaggttaaa cttttattta aataaaaacc
     9001 ctatgttaat ttggtttcaa tcgagtaaga tgagaagtca atcttttgat ggtagctagt
     9061 gatgttggaa atatagttgt gccagcttat ctagaccaag catgcacagc tggcgttctc
     9121 agccagatcc ccttgacagg atatattggc gggtaaacta agtcgctgta tgtgtttgtt
     9181 tgagatctca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9661 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9721 ccagttacct tcggaagaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9961 agttttaaat caatctaaag tatatatgtg taacattggt ctagtgatta tttgccgact
    10021 accttggtga tctcgccttt cacgtagtga acaaattctt ccaactgatc tgcgcgcgag
    10081 gccaagcgat cttcttgtcc aagataagcc tgcctagctt caagtatgac gggctgatac
    10141 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
    10201 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
    10261 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
    10321 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
    10381 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
    10441 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
    10501 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
    10561 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
    10621 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
    10681 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
    10741 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcataaca
    10801 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
    10861 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
    10921 acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg
    10981 gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt
    11041 ggctccctca ctttctggct ggatgatggg gcgattcagg cgatccccat ccaacagccc
    11101 gccgtcgagc gggct
//
