LOCUS       sequence-220887-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes ConL3 as a Type 1 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK005
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(538..557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcac gtttaggttg tctgtgccca tacagaactg
      181 agacggtact gggttcgagt tccctgatcc gtatgtcgaa tggctaccag gctgacgacg
      241 atgatatgag cgtgcatcgg catcttgcgg tgttgattag ctgccactgg tggctgtcct
      301 acatcagcct catgtggtct aggtctgctc tagatgcgaa ttcagggtga gaccgactac
      361 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      421 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      481 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      541 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      601 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      661 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      721 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      781 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      841 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
      901 cagtatttgg tatctgcgct ctgctgaagc cagttacctt c
//
