LOCUS       sequence-220889-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes ConL5 as a Type 1 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK007
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      48..82
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(484..503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
       61 gttgccgccg ggcgtttttt attggtctgg tctcacgttc gactgtgtgg agctttattc
      121 actttgagac gtatcattct gtggtcggcg cggctccatt cggctatgtc ttaaccgggt
      181 ctgtggtcat cggcactatt gacgctggcc cctcgacaat ggtgcggatc atgcatcgag
      241 ttgattgtcg catgagtgcc ctgaccttgt gcgttctaga tgcgaattca gggtgagacc
      301 gactacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      361 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      421 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      481 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      541 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      601 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      661 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      721 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      781 cgaggtatgt aggcggtgct acagagttct tgaagtggtg g
//
