LOCUS       sequence-220891-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pCCW12 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK010
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcac atagatcttt attgatatag tgtttaagcg
      181 aatgacagaa gattaatttc ttggtatgtt aggaaagaat aaaggagaat aagaataatt
      241 agaacaatgt aggatggaaa gaaagattat caagcatgcc gactttatat acttgaacgg
      301 aggcaaagga tgcaaaattt tctcacattt ctttctgccg ttatgttgga agtaagactc
      361 ccattatcgc aatactgcaa cacgaatatg caaaatttgc tgagttatcg cagatagttg
      421 ttgcaaagat agcggcgtag gtggccgcga aatgggaaat tccaaaacaa acggtttttt
      481 tactcctgag aaatacttgt acgggataat ccagggccta ccacccacgc ttcgaggatt
      541 ggcttttatt tttttttttt tggtggcgtt ttatttcttt cccgctttct gggacttgtg
      601 cggagttttg agaggggcgc gcggcaaagg attcccaaaa cggaaatcag acgccaatag
      661 ccagcactca aagcagttct ggacccattc cgattttccc atttggttct tgcgcgtgct
      721 gattccgaca cgcgcgtcta taaatagcat gaagtatccg cacaccgcag cgttagtgag
      781 gtgagggtgg cagcaagcta attcccgcat ctggaatctg aactgcccct tttggactaa
      841 ccgtgtggtt catgggtgcg tttgagaccg actacggtta tccacagaat c
//
