LOCUS       sequence-220896-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pPGK1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK011
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcac atagatcttg ttttatattt gttgtaaaaa
      181 gtagataatt acttccttga tgatctgtaa aaaagagaaa aagaaagcat ctaagaactt
      241 gaaaaactac gaattagaaa agaccaaata tgtatttctt gcattgacca atttatgcaa
      301 gtttatatat atgtaaatgt aagtttcacg aggttctact aaactaaacc acccccttgg
      361 ttagaagaaa agagtgtgtg agaacaggct gttgttgtca cacgattcgg acaattctgt
      421 ttgaaagaga gagagtaaca gtacgatcga acgaactttg ctctggagat cacagtgggc
      481 atcatagcat gtggtactaa accctttccc gccattccag aaccttcgat tgcttgttac
      541 aaaacctgtg agccgtcgct aggaccttgt tgtgtgacga aattggaagc tgcaatcaat
      601 aggatgacag gaagtcgagc gtgtctgggt tttttcagtt ttgttctttt tgcaaacaaa
      661 tcacgagcga cggtaatttc tttctcgata agaggccacg tgctttatga gggtaacatc
      721 aattcaagaa ggagggaaac acttcctttt tctggccctg ataatagtat gagggtgaag
      781 ccaaaataaa ggattcgcgc ccaaatcggc atctttaaat gcaggtatgc gatagttcct
      841 cactctttcc ttactcaccg tttgagaccg actacggtta tccacagaat c
//
