LOCUS       sequence-220900-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pRET2 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK020
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcac atagatcttg tgtatttctt ttgatggagc
      181 tataggtatt ctaatatgat ctatattcga cgctgaggct aattgccctt gttcttgagg
      241 tgtcaacaac cagattttct aatttttcgc tggctgttgc tggaaaaatg taaacgcgca
      301 gggcgggtaa taataaaagt tgaactatga agaacaatag aaacgccaag gtttactgac
      361 agagacatgt tttaattcaa gtgatgaggc ggaaacgtgc aagatcctaa atgaaggata
      421 aaaagagttc ttaaaaaggg aagtaaggaa taacagagta gaaaaaccga aaagacaact
      481 taacaaatcg gcaacacttt tatggggccc cgctcgcctg tgtgcaagta gtattcgacc
      541 tggaacacgc atttaccacg agatgacagc aatagtccgt acaacattaa ttagtttcga
      601 caattgctcg cctttataag ccatgctagt gcccaatcaa acactttact tgccctgaag
      661 ttcctttttt cgctagcctg taacttaaat aagccatcta accttttttt tctaaaaatt
      721 ttctttatta ccctgtcggc ttattttcta ttctacacat tatttgccac ccattgaaat
      781 tgtagcttgt attaataggg aaaagccgga agtataaccg gtggaaagta ctattgaagt
      841 gagataagaa gccatcgtcg tttgaga
//
