LOCUS       sequence-220902-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pMF_2 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK029
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcac atagatcttt tctccaatat gtgaatttac
      181 tggaatttga tgcaggtgat ggtagtaatt atccattata tagaatataa aaacagatat
      241 aggaaggaca cctcaaacta caccagaatc aaggacagct tttatacata taagccctca
      301 tctctgcgct gtaaagacac caagtttcac taggtccacg gcgactctgt actgaaacaa
      361 ctgaatattt gaaacagtaa cgatgaatag tttggctcga taccttgaaa acaatcacta
      421 caggcgtcgc ccagatgcgc acatcgttgc tggcattgat gtaccaatga agaaatataa
      481 gtgacaaact ttatgaaaag atagaaagag gtttacaaga taagagaaaa attatgctgt
      541 gaagattagt aatcgaagta actgcttatt cattcagtgc atgtttttct aggaatgcgt
      601 ttgatatggg acatacatct tactatgcga cattaccttt tagggacatc aatgagcaag
      661 tgaacactag caaattagga aaacaacttt agcacgccca actgcgtact gaaacgcaga
      721 aaactataga gtttcccgaa acggtgacag ctgagttcgc tcaaataaga acaacaccat
      781 gtcttgaagc ttcttctata gcgtagagta agtagtgcct tgtagcccta ccgttttaca
      841 atgcatgagg ttacccgcac ttattatttt tttctctttt tttttcttag ctataaaagg
      901 cagatcaacg tttgagaccg actac
//
