LOCUS       sequence-220906-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pGAL1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK030
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     promoter        complement(170..611)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(176..199)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    494..611
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcac atagatctta tagttttttc tccttgacgt
      181 taaagtatag aggtatatta acaatttttt gttgatactt ttatgacatt tgaataagaa
      241 gtaatacaaa ctgaaaatgt tgaaagtatt agttaaagtg gttatgcagc ttttccattt
      301 atatatctgt taatagatca aaaatcatcg cttcgctgat taattacccc agaaataagg
      361 ctaaaaaact aatcgcatta tcatcctatg gttgttaatt tgattcgtta atttgaaggt
      421 ttgtggggcc aggttactgc caatttttcc tcttcataac cataaaagct agtattgtag
      481 aatctttatt gttcggagca gtgcggcgcg aggcacatct gcgtttcagg aacgcgaccg
      541 gtgatgacga ggacgcacgg aggagagtca tccgtcggag ggctgtcgcc cgctcggcgg
      601 cttctaatcc gtacttcaat atagcaatga gcagttaagc gtattactga aagttccaaa
      661 gagaaggttt ttttaggcta agataatggg gcgtttgaga ccgactacgg ttatccacag
      721 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      841 aaaatcgacg ctcaagtcag a
//
