LOCUS       sequence-220907-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Venus as a Type 3 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK033
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(156..869)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcag gatcctttgt acaattcatc cataccatgg
      181 gtaataccag cagcagtaac aaattctaac aagaccatgt gatctctctt ttcgtttgga
      241 tctttggata aggcagattg ataggataag taatggttgt ctggtaacaa gactggacca
      301 tcaccaattg gagtattttg ttgataatgg tcagctaatt gaacaccacc atcttcaatg
      361 ttgtgtctaa ttttgaagtt agctttgata ccattctttt gtttgtcagc agtgatgtaa
      421 acattgtgag agttatagtt gtattccaat ttgtgaccta aaatgttacc accttcttta
      481 aaatcaatac cttttaattc gattctatta actaaggtat caccttcaaa cttgacttca
      541 gctctggtct tgtagttacc gtcatctttg aaaaaaatag ttctttcttg aacataacct
      601 tctggcatgg cagacttgaa aaagtcatgt tgtttcatat gatctgggta tctagcaaaa
      661 cattgcaaac cataacctaa agtagtgact aaggttggcc atggaactgg caatttacca
      721 gtagtacaaa tcaattttaa ggtcaattta ccgtaagtag catcaccttc accttcaccg
      781 gagacagaaa atttgtgacc attaacatca ccatctaatt caaccaaaat tgggacaaca
      841 ccagtgaata attcttcacc tttagacata tgagaccgac tacggttatc cacagaatca
      901 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      961 aaggccgcgt tgctggcg
//
