LOCUS       sequence-220908-        5869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa251 of Cre recombinase fused to nMag CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2693_pCAG-PV1-iCre-N251-L1-nMag-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1922..1942
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1952..2176
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2416..2435)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2481..2536
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2535..2554)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2897..2913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2897..2913)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2910..2932)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2921..2937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2945..2951,2952..2969,2970..2975))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2990..3011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3096..3230
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3250..3384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3287..3306)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3341..3360
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3452..3469)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3623..4211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3703..3722)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4382..5173,5174..5242))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5005..5024
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5243..5347)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5378..5757
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgt ccaacctgct gactgtgcac caaaacctgc ctgccctccc
      721 tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca
      781 ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg
      841 gtgcaagctg aacaacagga aatggttccc tgctgaacct gaggatgtga gggactacct
      901 cctgtacctg caagccagag gcctggctgt gaagaccatc caacagcacc tgggccagct
      961 caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg ctgtgtccct
     1021 ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca agcaggccct
     1081 ggcctttgaa cgcactgact ttgaccaagt cagatccctg atggagaact ctgacagatg
     1141 ccaggacatc aggaacctgg ccttcctggg cattgcctac aacaccctgc tgcgcattgc
     1201 cgaaattgcc agaatcagag tgaaggacat ctcccgcacc gatggtggga gaatgctgat
     1261 ccacattggc aggaccaaga ccctggtgtc cacagctggt gtggagaagg ccctgtccct
     1321 gggggttacc aagctggtgg agagatggat ctctgtgtct ggtgtggctg atgaccccaa
     1381 caactacctg ttctgccggg tcagaaagaa tggtgtggct gccccttctt ccggagggtc
     1441 tggctccgga tcaagtggtg gcagcggtac ccatactctt tatgcccccg gtggatatga
     1501 cattatggga tatctggacc agatcggcaa ccggccaaac ccgcaggtgg aactgggccc
     1561 cgtggataca tcctgcgcct tgattctttg tgacctgaaa cagaaagaca ccccgatagt
     1621 ttacgcgagt gaagccttcc tctacatgac aggttacagc aacgcagagg tgctgggccg
     1681 gaattgccgg tttctgcaaa gccctgacgg catggtgaag cccaagagca cccggaagta
     1741 cgtggatagt aacacaatca atactatgcg caaggcaatc gacaggaatg ccgaggtgca
     1801 ggttgaagta gtcaatttta aaaagaatgg acagcgattt gttaatttcc tgactatgat
     1861 acctgttagg gacgaaacag gcgagtatcg atactctatg ggattccagt gcgaaacaga
     1921 acccaagaaa aagcggaagg tgtgagaatt cctgtgcctt ctagttgcca gccatctgtt
     1981 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     2041 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     2101 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     2161 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     2221 cacgcgccct gtagcgtgta ccgctgggag ttcgtagacg gaaacaaacg cagaatccaa
     2281 gcgcggccgc ccaggataca tagattacca caactccgag cccttccacc gctagcgcac
     2341 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     2401 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     2461 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     2521 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     2581 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     2641 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     2701 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     2761 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     2821 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     2881 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2941 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3001 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     3061 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccagttc
     3121 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3241 caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3301 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3361 aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag
     3421 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3481 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3541 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3601 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3661 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3721 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3781 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3841 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3901 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3961 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4021 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4081 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4141 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4201 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4261 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4321 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4381 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4441 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4501 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4561 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     4621 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     4681 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     4741 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     4801 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     4861 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     4921 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     4981 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5041 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5101 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5161 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5221 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5281 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5341 ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat
     5401 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     5461 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     5521 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     5581 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     5641 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     5701 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     5761 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     5821 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcggggg
//
