LOCUS       sequence-220909-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mRuby2 as a Type 3 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK034
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(156..866)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcag gatcccttat acaattcatc cataccaccg
      181 cctaagccag caaatttggc tacggcatgc tctctctgta caacgaacat ttcgttatcg
      241 ctctcttcga gtctttctag tctgtggtct acagcatgta ttcctggcat tttgatattg
      301 ccaacagtct ttttagaacg ataagtggta acaaaactac aagacaaatg acctccacca
      361 tcaactttaa gcgccatgtg tgtgtagcct ctcaaacctc catcagctgg gtacatcatt
      421 tcagtatttg gttcccaacc tttggtcttt ttctgcatca caggtccgtt agaaggaaaa
      481 ttcacccctc ttacttgcac atgatagaca aggcagccat cttctaatga agtatcttgc
      541 ataactgtga cgacaccccc atcttcgtat cttgtaaccc tttcccatgt aaaaccctct
      601 ggaaaagatt gcttgaaaaa gtctggtata cctttagggt acttgatgaa agttcgtgat
      661 ccgtacatga atgacgttgc cagaatgtca aatgcaaatg gtagtggtcc gccttcaatt
      721 actttaattc tcatagtttg tgtacccatg taaggattac cttcaccttc ccctgtacac
      781 ttgaattggt gaccattaac ggagccctcc atgacaactt tcattctcat gttttccttg
      841 attaactcct ctcctttgga caccatatga gaccgactac ggttatccac agaatcacgg
      901 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgt
//
