LOCUS       sequence-220910-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes I-SceI (ORF) as a Type 3 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK035
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcag gatccttact tcaagaaggt ttcagaagag
      181 atggtgtttg gcaacttgta catcatttgt gggatcaagt atggcttgat caagttgtag
      241 aagatcaagt aagacataga gtcgatgtag atgattggct tgttcttgtt gatcttaacg
      301 taacagttca attggaactt gtttctcaaa cccttaacca agtattcaac ttcttcgaag
      361 gtgaaagatt gggtgttcaa aacgatagac ttgttggtag agttcttgtt gtagtcccac
      421 ttaccaccgt cgtccatgaa ccagtaagcc aaagacattg gggtcaagta gttttcaacc
      481 aagttgtttg ggatggtttt cttgttgtta acgatgaaca agttagccaa cttgttgaaa
      541 gcttggtgct tgaaggtttg agcaccccag gtgataacca agttacccaa gtggttaact
      601 ctttccttct tgtgtggtgg agacaaaacc cattggtcgt acaacaaaca aacgtggtcc
      661 atgtaagcct tgttcttcca ttcgaattgc atacagtagg tcttaccttc gtcgcgagat
      721 cggatgtaag cgtcacccaa gatcaaaccg ataccagctt cgaattgttc gatgttcaat
      781 tcgatcaatt gagacttgta ttccttcaac aacttagagt ttggacccaa gttcataact
      841 tggttcttct tgatgttctt catatgagac cgactacggt tatccacaga atcaggggat
      901 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg t
//
