LOCUS       sequence-220916-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes 6XHis_3XFlag as a Type 3a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK040
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             123..194
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             198..215
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(235..269)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
       61 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcata
      121 tggattacaa ggacgatgat gataaggact acaaagatga tgatgacaaa gattataagg
      181 acgatgatga taaaggtcat catcatcatc atcacggttc ttgagaccag accaataaaa
      241 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
      301 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
      361 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
      421 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
      481 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
      541 gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa
      601 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
      661 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
      721 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
      781 aaattccgga tgagcattca tca
//
