LOCUS       sequence-220917-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Ubi-M as a Type 3a part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK041
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      80..114
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(254..481)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(673..692)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggggcgtaat ttgatatcga gctcgcttgg actcctgttg atagatccag taatgacctc
       61 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtct
      121 ggtctcaaga acctgatctc tgcattaaag agtctgccaa ggctcttgga gaggcagttt
      181 gagtatttgg tgccaatgtg gtctttcttt taaccaatga tacaggtaac aaccaggcac
      241 cagaaccatg catacctccc cttaatctta agactaagtg caaagtagat tctttctgta
      301 tgttataatc agacaaagtc ctaccgtcct ccaactgctt acctgcgaaa atcaatcttt
      361 gttgatctgg aggaatacct tccttatcct ggatcttaga tttaacatta tcgatagtat
      421 cagatgactc aacttctaaa gtaattgttt taccagttaa agttttaacg aaaatctgca
      481 tatgagaccg actacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      541 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      601 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      661 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      721 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      781 tttctcatag ctcacgctgt aggtatctca
//
