LOCUS       sequence-220921-       14541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14541)
  TITLE     Doench CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pXPR_118
FEATURES             Location/Qualifiers
     source          1..14541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        29..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        410..608
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             626..806
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    853..978
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1471..1704
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1889..1933
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2231..2348
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2478..3656
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2709..3647
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3604..3624
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3672..3719
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(3720..3743,3744..3746,3747..6302,6303..6305,
                     6306..7820)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7875..7895
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7920..7973
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7974..8999
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVHRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    9024..9612
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9077..9097)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9495..9506)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9615..9631)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9616..9632)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10137..10317
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10343..10360)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10349..10573
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10619..11047
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10706..10725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10916..10937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11056..11076)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11061..11390
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11241..11376
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11303..11322
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11438..11485
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11504..11878
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12008..12129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12045..12064)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12099..12118
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12178..12194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12178..12194)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12191..12213)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12202..12218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12226..12232,12233..12250,12251..12256))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12271..12292
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12409..12426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12580..13168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12660..12679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13339..14130,14131..14199))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13962..13981
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14200..14304)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(14379..14398)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
      661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      781 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
      841 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      901 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      961 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     1021 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1081 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1141 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1201 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1261 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1321 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     1381 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     1441 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     1501 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1561 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1621 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     1681 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     1741 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     1801 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     1861 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     1921 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     1981 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     2041 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     2101 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     2161 cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat
     2221 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     2281 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     2341 aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt agctagctgc
     2401 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
     2461 tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     2521 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     2581 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2641 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2701 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     2761 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     2821 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     2881 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     2941 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     3001 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     3061 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     3121 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     3181 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     3241 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3301 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3361 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3421 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3481 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3541 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3601 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacgta
     3661 cggccaccat gaaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
     3721 acaagaagta cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca
     3781 ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca
     3841 gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca gccgaggcca
     3901 cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc
     3961 tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg
     4021 aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca
     4081 tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac
     4141 tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga
     4201 tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg
     4261 acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca
     4321 acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc
     4381 tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga
     4441 ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg
     4501 ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga
     4561 tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc
     4621 tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga
     4681 tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc
     4741 agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct
     4801 acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa
     4861 agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc
     4921 agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca
     4981 ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga
     5041 agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat
     5101 tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg
     5161 tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc gataagaacc
     5221 tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtata
     5281 acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg
     5341 gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga
     5401 agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg
     5461 gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca
     5521 aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga
     5581 ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc
     5641 tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc
     5701 tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt
     5761 tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc
     5821 tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg
     5881 agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga
     5941 aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg
     6001 aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga
     6061 agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg
     6121 aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata
     6181 tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gaccacatcg
     6241 tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc agaagcgaca
     6301 aggcccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact
     6361 actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac aatctgacca
     6421 aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg
     6481 tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta
     6541 agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg aagtccaagc
     6601 tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc
     6661 accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc
     6721 ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga
     6781 tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca
     6841 tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc
     6901 tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg gattttgcca
     6961 ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga
     7021 caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag ctgatcgcca
     7081 gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt
     7141 ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag
     7201 agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc
     7261 tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact
     7321 ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga
     7381 agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact
     7441 atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc
     7501 acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc
     7561 tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg gataagccca
     7621 tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg
     7681 ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg
     7741 tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc
     7801 tgtctcagct gggaggcgac agcgctggag gaggtggaag cggaggagga ggaagcggag
     7861 gaggaggtag cggacctaag aaaaagagga aggtggcggc cgctggatcc ggcagtggag
     7921 agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccaatgaaaa
     7981 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtgt
     8041 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     8101 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     8161 ttcatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     8221 ttagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     8281 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     8341 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     8401 aatacactac gtggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     8461 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     8521 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     8581 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     8641 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     8701 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     8761 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     8821 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     8881 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     8941 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataag
     9001 aattcgatat caagcttatc ggtaatcaac ctctggatta caaaatttgt gaaagattga
     9061 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     9121 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     9181 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     9241 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     9301 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     9361 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     9421 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     9481 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     9541 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     9601 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     9661 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     9721 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     9781 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9841 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     9901 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     9961 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
    10021 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
    10081 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
    10141 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    10201 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    10261 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
    10321 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
    10381 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
    10441 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
    10501 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
    10561 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
    10621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    10681 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    10741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    10801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    10861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    10921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    10981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    11041 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
    11101 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
    11161 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
    11221 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    11281 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
    11341 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
    11401 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
    11461 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
    11521 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
    11581 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
    11641 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
    11701 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
    11761 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
    11821 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
    11881 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    11941 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    12001 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    12061 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    12121 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    12181 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    12241 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    12301 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    12361 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    12421 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    12481 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    12541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    12601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    12661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    12721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    12781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    12841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    12901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    12961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    13021 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    13081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    13141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    13201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    13261 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    13321 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    13381 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    13441 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    13501 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    13561 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    13621 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    13681 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    13741 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    13801 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    13861 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    13921 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    13981 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    14041 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    14101 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    14161 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    14221 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    14281 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    14341 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    14401 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    14461 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
    14521 gttaggcgtt ttgcgctgct t
//
