LOCUS       sequence-220922-        9879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector for lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9879)
  TITLE     Doench CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLX_TRC311
FEATURES             Location/Qualifiers
     source          1..9879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     290..307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    424..445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(460..465,466..483,484..490)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    498..514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     503..525
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     522..538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     522..538
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     557..577
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        559..577
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        605..831
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2370..2580
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2392..2588
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2439..2574
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2610..3005
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3079..3196
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3334..4512
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3565..4503
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4460..4480
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4532..4656
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4681..4686,4687..4704,4705..4711)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4765..5424
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4831..4850)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5766..6071
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5950..5969
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6112..6236)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6244..6285
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6336..6924
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6389..6409)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6807..6818)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6996..7229
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7307..7428
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7344..7363)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7398..7417
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7468..7603
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7623..7642)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7624..7642)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7652..7669)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7652..7668)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7661..7683)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7810..8265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7897..7916)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8107..8128
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8291..8395
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8396..8464,8465..9256)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8614..8633)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9427..9879,1..136)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
       61 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      301 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      361 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      421 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      481 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
      541 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
      601 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
      661 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      721 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      781 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     2341 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     2401 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     2461 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2521 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2581 tttgcaaaaa gctttctaga ggtaccacca tggccaagcc tttgtctcaa gaagaatcca
     2641 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     2701 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     2761 ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     2821 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     2881 gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     2941 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     3001 agggcctgca gctgcagtag taaggcgcgc cgttaacgaa ttctagatct tgagacaaat
     3061 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     3121 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     3181 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     3241 cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     3301 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     3361 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     3421 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     3481 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     3541 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     3601 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     3661 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     3721 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     3781 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     3841 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     3901 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     3961 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     4021 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     4081 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     4141 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     4201 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     4261 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     4321 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     4381 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     4441 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     4501 caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag ctgaacgaga
     4561 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     4621 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
     4681 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     4741 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     4801 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     4861 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     4921 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     4981 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     5041 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     5101 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     5161 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     5221 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     5281 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     5341 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     5401 gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag
     5461 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     5521 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
     5581 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     5641 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     5701 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     5761 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     5821 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     5881 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     5941 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     6001 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     6061 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
     6121 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     6181 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttgat
     6241 atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgaactagt
     6301 accggttaag tcgacaatca acgcgttaag tcgacaatca acctctggat tacaaaattt
     6361 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     6421 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     6481 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6541 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6601 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6661 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6721 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     6781 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6841 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6901 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     6961 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7021 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7081 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7141 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7201 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     7261 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7321 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7381 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     7441 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     7501 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7561 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     7621 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     7681 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     7741 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     7801 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     7861 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     7921 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     7981 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     8041 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     8101 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     8161 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8221 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     8281 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8341 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8401 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8461 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8521 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8581 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8641 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8701 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8761 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8821 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8881 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8941 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     9001 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9061 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9121 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9181 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9241 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9301 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9361 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9421 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9481 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9541 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     9601 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9661 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9721 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9781 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9841 cgaagggaga aaggcggaca ggtatccggt aagcggcag
//
