LOCUS       sequence-220923-        6675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading
            frame and 500 nucleotides of the 3' UTR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Yao Z, Delorme-Axford E, Backues SK, Klionsky DJ
  TITLE     Atg41/Icy2 regulates autophagosome formation.
  JOURNAL   Autophagy. 2015;11(12):2288-99. doi: 10.1080/15548627.2015.1107692.
  PUBMED    26565778
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2015"; volume: "11(12):2288-99. doi"; pages: " 
            10.1080/15548627.2015.1107692"
COMMENT     Sequence Label: pRS414-ATG41
FEATURES             Location/Qualifiers
     source          1..6675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    83..104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(119..124,125..142,143..149)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    157..173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     162..184
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     181..197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     181..197
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     216..236
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        218..236
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     273..289
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     273..289
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2248..2267)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2249..2267)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2277..2294)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2277..2293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2286..2308)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2434..2889
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2521..2540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2731..2752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2992..3666)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(3667..3947)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(3968..3987)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4087..4109
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4147..4165)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    4206..4709
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        4746..4850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4851..4919,4920..5711)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5069..5088)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5882..6470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6371..6390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6624..6641
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
       61 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      121 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      181 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
      241 aaaagctgga gctccaccgc ggtggcggcc gctctagaac tagtggatcc cccgggctgc
      301 aggaattcct gcctctcgtc gtcattaaac agcgtgaggg ggccacagtc aagcaaatcc
      361 aactccgcca caagaccgag tgctattttc catggctctc ccccaaagct agttaataaa
      421 aaataagtaa caaatcatta tagtaattct taatcgatat ctatttaaga agtgtaacag
      481 cacatgcaat gaaccgactt cgtcattcat tacctctgct tttccctttt tcaacagtca
      541 cgtgcgcatc tcccttccgg gtaagcaccg cacatgccaa agaccctatc ctggctcgcc
      601 cactcactca tctccattag agactcaacc acatttgtgg gtgtgttaca gaagttacac
      661 catacccact gtcctggtcc gcaacagttg caaaatcaat gatgcctgct accgtcatgc
      721 tatcatgaca aacgatggca cctttgccaa gcaaaaaact gtaaacaaaa ccaaaccaag
      781 aagtcccttg gggttgtgag ccatattctc catctgcgtt gtttcataac cagcccaaag
      841 ggttgcacgt tctcttccgt cgatctggaa ccaagctgtg gcgctgcgtg gaaccgtccg
      901 gaaatctaca gaagccgtta gaagaaaaca gaataaacac acgtgacccc gtcagtgtgg
      961 aaagccgcgg gatcaccacc gttattcgtt ttttttagca cgcgcgcgaa gatgacacat
     1021 tgcacaccac atgtttatga ccattgttat tactcatttc actgttattt tgcatccaca
     1081 tccacacccg ggtggttttg caattcggta attactccac ggtgcataaa gaagcggctc
     1141 tgcgtaaaaa ggtatatata agaacaggac tcatcttgat ttcggaaagg gtctggagca
     1201 actctctttc tttcttactt gttttattct tgagaaaacc aaactaaatt ggctacacca
     1261 aaccaaccaa acttacacac aataatatac aattacaatt atttacaatg tcctctgtgg
     1321 aatcttcccc catctcgcgc tatgaagacg aggtcttccc tctttcgttt tcaaacgttg
     1381 ctttcgagcc ccctatgctc tcgcatagcc cggacagatc gacttatgcc gatgactttt
     1441 ctcaatctta ccagcaagaa ttgctaactt ttccgctatc gtacccgatt gtcgatgagt
     1501 cagaatgcac gcacactaaa gataagacgg acagcaacat aataacgagt actgaagacg
     1561 attgcatgtt cgatatggaa tttaacggca acgccgccag tgcagttgct gctgctagta
     1621 aggaatctaa ctctgcctct gggtttgcct ttgcaagtaa cgatgccttt gccaatgtcg
     1681 cacaacagaa ctacagactg tggttgtcat cggtatgacg cattttacca catctctact
     1741 tgttctcacg accccttgca tcgcatatcc tacacatttt aaatcggcat tccttccaag
     1801 catattctta attcctaatt ttacatagnt tttttttttt ttttagagaa aataacaaca
     1861 atatacatag aacttactta ctcaattcta ctatatcttt tttttttgtt actattagtc
     1921 atgtttgcat gactccatta cattttcgtt tttttgctag ccaattctgg tcgtcggaaa
     1981 aacttttcat tgaatctttc aaagcgtgta gcagttgcat ttttttaaaa atgaatatgt
     2041 atgcaaaata tatatatgta tatatatgcc taatatacat aataatagaa aaaaaaaacc
     2101 tcatacgaaa taggcgcttc tcgtatctat actcaacccg cccccaatgc tgaaaattgt
     2161 tactcgggct ggacacacag ctagaatatc gaacatcgca gcacatcttt tacgcacctc
     2221 tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     2281 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     2341 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2401 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
     2461 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2521 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2581 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2641 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     2701 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     2761 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     2821 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     2881 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     2941 agatcggcaa gtgcacaaac aatacttaaa taaatactac tcagtaataa cctatttctt
     3001 agcatttttg acgaaatttg ctattttgtt agagtctttt acaccatttg tctccacacc
     3061 tccgcttaca tcaacaccaa taacgccatt taatctaagc gcatcaccaa cattttctgg
     3121 cgtcagtcca ccagctaaca taaaatgtaa gctttcgggg ctctcttgcc ttccaaccca
     3181 gtcagaaatc gagttccaat ccaaaagttc acctgtccca cctgcttctg aatcaaacaa
     3241 gggaataaac gaatgaggtt tctgtgaagc tgcactgagt agtatgttgc agtcttttgg
     3301 aaatacgagt cttttaataa ctggcaaacc gaggaactct tggtattctt gccacgactc
     3361 atctccatgc agttggacga tatcaatgcc gtaatcattg accagagcca aaacatcctc
     3421 cttaggttga ttacgaaaca cgccaaccaa gtatttcgga gtgcctgaac tatttttata
     3481 tgcttttaca agacttgaaa ttttccttgc aataaccggg tcaattgttc tctttctatt
     3541 gggcacacat ataataccca gcaagtcagc atcggaatct agagcacatt ctgcggcctc
     3601 tgtgctctgc aagccgcaaa ctttcaccaa tggaccagaa ctacctgtga aattaataac
     3661 agacatactc caagctgcct ttgtgtgctt aatcacgtat actcacgtgc tcaatagtca
     3721 ccaatgccct ccctcttggc cctctccttt tcttttttcg accgaattaa ttcttaatcg
     3781 gcaaaaaaag aaaagctccg gatcaagatt gtacgtaagg tgacaagcta tttttcaata
     3841 aagaatatct tccactactg ccatctggcg tcataactgc aaagtacaca tatattacga
     3901 tgctgttcta ttaaatgctt cctatattat atatatagta atgtcgtgat ctatggtgca
     3961 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     4021 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     4081 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     4141 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     4201 agacggatcg cttgcctgta acttacacgc gcctcgtatc ttttaatgat ggaataattt
     4261 gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat
     4321 aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt
     4381 caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat taaatagata
     4441 tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta
     4501 cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagagc aagataaaag
     4561 gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt
     4621 tttctttaat ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt
     4681 aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa
     4741 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4801 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4861 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4921 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4981 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5041 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5101 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5161 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5221 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5281 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5341 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5401 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5461 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5521 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5581 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5641 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5701 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5761 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5821 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5881 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5941 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6001 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6061 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6301 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6361 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6421 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6481 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6541 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6601 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6661 tacgcaaacc gcctc
//
