LOCUS       sequence-220929-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes GFP dropout as a Type 234r part to be used in the Dueber YTK
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK047
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             262..273
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
       61 tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggaacgtgag accgaaagtg
      121 aaacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata atgtgtgcgg
      181 caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag aaacggcagg
      241 ttcggatctt agctactaga gaaagaggag aaatactaga tgcgtaaagg cgaagagctg
      301 ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg tcataagttt
      361 tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc
      421 tgtactactg gtaaactgcc ggttccttgg ccgactctgg taacgacgct gacttatggt
      481 gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc
      541 atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa
      601 acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc
      661 attgacttta aagaggacgg caatatcctg ggccataagc tggaatacaa ttttaacagc
      721 cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt
      781 cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca
      841 atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg
      901 tct
//
