LOCUS       sequence-220930-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes sgRNA Dropout as a Type 234 part to be used in the Dueber 
            YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK050
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     misc_RNA        complement(233..308)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(322..349)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(365..436)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcac agctatccac tagacagaag tttgcgttcc
      181 atactgtcag aggtggcatt ttacataaca atagtgacaa aaaataaaaa aagcaccgac
      241 tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc
      301 tctaaaactg agacgtataa acgcagaaag gcccacccga aggtgagcca gtgtgactct
      361 agtagagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      421 cctttcgttt tatttgatgc ctggtcattt gtacagttca tccataccat gcgtgatgcc
      481 cgctgcggtt acgaactcca gcagaaccat atgatcgcgt ttctcgttcg gatctttaga
      541 cagaacgctt tgcgtgctca gatagtgatt gtctggcagc agaacaggac catcaccgat
      601 tggagtgttt tgctggtagt gatcagccag ctgcacgctg ccatcctcca cgttgtggcg
      661 aattttaaaa ttcgctttaa tgccattttt ttgtttatcg gcggtgatgt aaacattgtg
      721 gctgttaaaa ttgtattcca gcttatggcc caggatattg ccgtcctctt taaagtcaat
      781 gcctttcagc tcaatgcggt ttaccagggt atcgccttca aatttcactt ccgcacgcgt
      841 tttgtacgtg ccgtcatcct taaaggaaat cgtgcgttcc tgcacatagc cttccggcat
      901 ggcggacttg aagaagtcat gctgcttcat atggtccgga taacgagcaa agcactgaac
      961 accataagtc agcgtcgtta ccaga
//
