LOCUS       sequence-220936-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes tADH1 as a Type 4 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK053
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      132..319
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(187..211)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(374..408)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
       61 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcaat
      121 cctaactcga ggcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa
      181 aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct
      241 tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta
      301 ttgaccacac ctctaccggc atgccgagca aatgcctgca aatcgctccc catttcgctg
      361 tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
      421 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
      481 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
      541 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
      601 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
      661 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct
      721 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
      781 atatgtgtag aaactgccgg aaatcgtcgt gg
//
