LOCUS       sequence-220938-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes mRuby2 as a Type 4a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK059
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(57..761)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="SKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMRI
                     KVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVV
                     TVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHMA
                     LKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAK
                     FAGLGGGMDELYK"
ORIGIN
        1 aacgctcggt tgccgccggg cgttttttat tggtctggtc tcagccactc gagttactta
       61 tacaattcat ccataccacc gcctaagcca gcaaatttgg ctacggcatg ctctctctgt
      121 acaacgaaca tttcgttatc gctctcttcg agtctttcta gtctgtggtc tacagcatgt
      181 attcctggca ttttgatatt gccaacagtc tttttagaac gataagtggt aacaaaacta
      241 caagacaaat gacctccacc atcaacttta agcgccatgt gtgtgtagcc tctcaaacct
      301 ccatcagctg ggtacatcat ttcagtattt ggttcccaac ctttggtctt tttctgcatc
      361 acaggtccgt tagaaggaaa attcacccct cttacttgca catgatagac aaggcagcca
      421 tcttctaatg aagtatcttg cataactgtg acgacacccc catcttcgta tcttgtaacc
      481 ctttcccatg taaaaccctc tggaaaagat tgcttgaaaa agtctggtat acctttaggg
      541 tacttgatga aagttcgtga tccgtacatg aatgacgttg ccagaatgtc aaatgcaaat
      601 ggtagtggtc cgccttcaat tactttaatt ctcatagttt gtgtacccat gtaaggatta
      661 ccttcacctt cccctgtaca cttgaattgg tgaccattaa cggagccctc catgacaact
      721 ttcattctca tgttttcctt gattaactcc tctcctttgg a
//
