LOCUS       sequence-220940-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes tENO1 as a Type 4b part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK061
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(573..592)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcac agcatacatg ggtgaccaaa agagcgggcg
      181 gatacacgcg tcaccacaag cagaataaaa ggtaaacctg aaattgtttt aacataaaat
      241 gaaaaatgct tgtttgcaac cctatataga atcataaaac attcgtgact ataaaatgaa
      301 taaactaaac tattctaaga aaatgaaata aatgacaaaa aaacgtgttt tttggactag
      361 aaggcttaat caaaagctgc caggagaccg actacggtta tccacagaat caggggataa
      421 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      481 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      541 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      601 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      661 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      721 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      781 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      841 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      901 gaagtggtgg cctaactacg gctacactag aagaa
//
