LOCUS       sequence-220941-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes tADH1 as a Type 4b part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK063
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      122..309
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(177..201)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(364..398)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 tatggaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
       61 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcatgg
      121 cgcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
      181 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
      241 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac
      301 ctctaccggc atgccgagca aatgcctgca aatcgctccc catttcgctg tgagaccaga
      361 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
      421 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
      481 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
      541 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
      601 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
      661 aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc
      721 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
      781 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
      841 atggaaaacg gtgtaacaag ggtgaacact at
//
