LOCUS       sequence-220942-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes tTDH1 as a Type 4b part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK066
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(325..359)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
       61 ataaccgtag tcggtctcct ggcataaagc aatcttgatg aggataatga tttttttttg
      121 aatatacata aatactaccg tttttctgct agattttgtg atgacgtaaa taattacata
      181 ttacttttta agccaagaca agattaagca ttaactttac ccttttcttt ctaagtttca
      241 atattagtta tcactgttta aaagttatgg cgagaacgtc ggcggttaaa atatattacc
      301 ctgaacggct gtgagaccaa accaataaaa aacgcccggc ggcaaccgag cgttctgaac
      361 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
      421 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
      481 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
      541 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
      601 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa
      661 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
      721 atcttg
//
