LOCUS       sequence-220944-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes SpecR-ColE1 as a Type 8 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK085
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    131..149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    156..174
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             192..203
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             210..887
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
       61 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgtg cggccgcccc
      121 ttgagaccag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
      181 gcacggatct gaaagaggag aaaggatcta tggcgagtag cgaagacgtt atcaaagagt
      241 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
      301 gtgaaggtga aggtcgtccg tacgaaggta ctcagaccgc taaactgaaa gttaccaaag
      361 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
      421 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
      481 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
      541 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtact aacttcccgt
      601 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
      661 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
      721 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
      781 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
      841 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa ggatctccag
      901 gcatcaaata aaacgaaagg
//
