LOCUS       sequence-220945-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes URA3 3' Homology as a Type 7 part to be used in the Dueber 
            YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK086
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(868..887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcat cggtctttat atttacatgc taaaaatggg
      181 ctacaccaga gatacataat tagatatata tacgccagta caccttatcg gcccaagcct
      241 tgtcccaagg cagcgttttg ttcttggaaa cgctgcccta cacgttcgct atgcttcaag
      301 aacttttctg agcacttcat gatgcatgtt tgttccttat tggttagctt tgatgttgtg
      361 aagtcattga cacagtctgt gaaacatctt tctaccagat tagagtacaa acgcatgaaa
      421 tccttcattt gcttttgttc cactactttt tggaactctt gttgttcttt ggagttcaat
      481 gcgtccatct ttacagtcct gtcttattgt tcttgatttg tgccccgtaa aatactgtta
      541 cttggttctg gcgaggtatt ggatagttcc tttttataaa ggccatgaag ctttttcttt
      601 ccaatttttt ttttttcgtc attatagaaa tcattacgac cgagattccc ggggcagtgg
      661 attcaagtgc tctactctga gaccgactac ggttatccac agaatcaggg gataacgcag
      721 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      781 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      841 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct g
//
