LOCUS       sequence-220946-       10961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Target-AID UGI-LVA vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Banno S, Nishida K, Arazoe T, Mitsunobu H, Kondo A
  TITLE     Deaminase-mediated multiplex genome editing in Escherichia coli.
  JOURNAL   Nat Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 
            10.1038/s41564-017-0102-6.
  PUBMED    29403014
REFERENCE   2  (bases 1 to 10961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2018 Feb 5. pii: 10.1038/s41564-017-0102-6. doi: 
            10.1038/s41564-017-0102-6."
COMMENT     Sequence Label: pScI_dCas9-CDA-UL
FEATURES             Location/Qualifiers
     source          1..10961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1905..1927
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1919..1936
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1920..1936
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1976..2227)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(2234..2857)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             complement(2864..2929)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(2948..3013)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(join(3221..4804,4805..4807,4808..7294,7295..7297,
                     7298..7324))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             7430..7438
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7443..8156
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAEMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     CDS             complement(8285..8944)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8859..8878
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(8998..9004,9005..9022,9023..9028))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             complement(9192..10142)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTMKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      10190..10412
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     10504..10522
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10560..10582)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10682..10701
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgacgcttta tatagaaaag aagattcaac taggtaaaat cttaatatag gttgagatga
       61 taaggtttat aaggaatttg tttgttctaa tttttcactc attttgttct aatttctttt
      121 aacaaatgtt cttttttttt tagaacagtt atgatatagt tagaatagtt taaaataagg
      181 agtgagaaaa agatgaaaga aagatatgga acagtctata aaggctctca gaggctcata
      241 gacgaagaaa gtggagaagt catagaggta gacaagttat accgtaaaca aacgtctggt
      301 aacttcgtaa aggcatatat agtgcaatta ataagtatgt tagatatgat tggcggaaaa
      361 aaacttaaaa tcgttaacta tatcctagat aatgtccact taagtaacaa tacaatgata
      421 gctacaacaa gagaaatagc aaaagctaca ggaacaagtc tacaaacagt aataacaaca
      481 cttaaaatct tagaagaagg aaatattata aaaagaaaaa ctggagtatt aatgttaaac
      541 cctgaactac taatgagagg cgacgaccaa aaacaaaaat acctcttact cgaatttggg
      601 aactttgagc aagaggcaaa tgaaatagat tgacctccca ataacaccac gtagttattg
      661 ggaggtcaat ctatgaaatg cgattaagct ttttctaatt cacataagcg tgcaggttta
      721 aagtacataa aaaatataat gaaaaaaagc atcattatac taacgttata ccaacattat
      781 actctcatta tactaattgc ttattccaat ttcctattgg ttggaaccaa caggcgttag
      841 tgtgttgttg agttggtact ttcatgggat taatcccatg aaacccccaa ccaactcgcc
      901 aaagctctat attgataaaa ataataatag tgggtataat taagttgtta gagaaaacgt
      961 ataaattagg agggattcat gctggtatga acaaaaatat aaaatattct caaaactttt
     1021 taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata
     1081 ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta
     1141 aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa
     1201 aactgaatac tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca
     1261 aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa
     1321 aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca
     1381 agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc
     1441 agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct
     1501 taataaaact tacccgccat accacagatg ttccagataa atattggaag ctatatacgt
     1561 actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc
     1621 atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat
     1681 tgtctatttt taatagttat ctattattta acgggaggaa ataaatgcat aaactgcatc
     1741 ccttaacttg tttttcgtgt acctattttt tgtgaatcgc cattcgccat tcaggctgcg
     1801 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     1861 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     1921 taaaacgacg gccagtgaat tcgagctcgg tacccggccg ttatgcaacc agtcctagca
     1981 tcttgatctt gttctctcca ttagagtcct gaatcaccag agcccagggt ttgtactctg
     2041 gggcatctga cgtgagcagc atcacgttct catcggtgct ctcatcatac gcagtgtgta
     2101 cgagaatgtc actttccggc ttattcccaa tgacctcctc aacttcttca ggaagcatga
     2161 gtatcgactc ttggatgacc aactgtttgc ctgtttcctt ctctatgatg tcggaaaggt
     2221 tggtcattct agaaacagca ggactcttag tggtgtggag tatttttacc tgaatcataa
     2281 tggacaactc gctccgtcgt ttttcagctc gcttcaaagt cttctcaagc catctattct
     2341 cattcaattg attgtgcgac gattggatga atattttcct gcaacattgg tagtgttcac
     2401 ttaccattac attcaaccca accccgttat ctctgaggtt ccacagccca atttgattcc
     2461 tcgcattttt ctcgtaatag agtttgcaag cccagatttt caaagtgtgg ccgttccccc
     2521 gcagctcctg gttataccat tctaagatct tttcagcgca atctgcacaa ggactccagg
     2581 atgagtacca atttatcgtg aattgtccgg ggttgtcgcg caggtattct tcgacttttc
     2641 taatgctaaa gatttcggcg tgaatgccac gttctgtccc gctctgtggt ttattcacag
     2701 catagcccca aaaacacgct ctacgttcac cccgtcgttt taattcaaag agaacgtagc
     2761 atctatgcga cacggatttt ttgttgttga aaaactgttt cttaaacgtg tagatgtcca
     2821 acttctcatg gattctcacg tactcagcgt cggtcatcct agacttatcg tcatcgtctt
     2881 tgtaatcaat atcatgatcc ttgtagtctc cgtcgtggtc cttatagtct ccggactcga
     2941 gcctagactt atcgtcatcg tctttgtaat caatatcatg atccttgtag tctccgtcgt
     3001 ggtccttata gtctccggaa tacttctcca cgtaagggac aggaatcatc cccctctttc
     3061 cttcgctgtc ctctgcattc caccactgct cctcaggctt atcccggatt ctcaggatgt
     3121 ctcctttctt aaagggaaga tcctcttcat cattcccatt aaagtcaaag agggctcgca
     3181 catactcagc agaacctcca cctccagaac ctcctccacc gtcacctcct agctgactca
     3241 aatcaatgcg tgtttcataa agaccagtga tggattgatg gataagagtg gcatctaaaa
     3301 cttcttttgt agacgtatat cgtttacgat caattgttgt atcaaaatat ttaaaagcag
     3361 cgggagctcc aagattcgtc aacgtaaata aatgaataat attttctgct tgttcacgta
     3421 ttggtttgtc tctatgtttg ttatatgcac taagaacttt atctaaattg gcatctgcta
     3481 aaataacacg cttagaaaat tcactgattt gctcaataat ctcatctaaa taatgcttat
     3541 gctgctccac aaacaattgt ttttgttcgt tatcttctgg actacccttc aacttttcat
     3601 aatgactagc taaatataaa aaattcacat atttgcttgg cagagccagc tcatttcctt
     3661 tttgtaattc tccggcacta gccagcatcc gtttacgacc gttttctaac tcaaaaagac
     3721 tatatttagg tagtttaatg attaagtctt ttttaacttc cttatatcct ttagcttcta
     3781 aaaagtcaat cggatttttt tcaaaggaac ttctttccat aattgtgatc cctagtaact
     3841 ctttaacgga ttttaacttc ttcgatttcc ctttttccac cttagcaacc actaggactg
     3901 aataagctac cgttggacta tcaaaaccac catatttttt tggatcccag tcttttttac
     3961 gagcaataag cttgtccgaa tttctttttg gtaaaattga ctccttggag aatccgcctg
     4021 tctgtacttc tgttttcttg acaatattga cttggggcat ggacaatact ttgcgcactg
     4081 tggcaaaatc tcgcccttta tcccagacaa tttctccagt ttccccatta gtttcgatta
     4141 gagggcgttt gcgaatctct ccatttgcaa gtgtaatttc tgttttgaag aagttcatga
     4201 tattagagta aaagaaatat tttgcggttg ctttgcctat ttcttgctca gacttagcaa
     4261 tcattttacg aacatcataa actttataat caccatagac aaactccgat tcaagttttg
     4321 gatatttctt aatcaaagca gttccaacga cggcatttag atacgcatca tgggcatgat
     4381 ggtaattgtt aatctcacgt actttataga attggaaatc ttttcggaag tcagaaacta
     4441 atttagattt taaggtaatc actttaacct ctcgaataag tttatcattt tcatcgtatt
     4501 tagtattcat gcgactatcc aaaatttgtg ccacatgctt agtgatttgg cgagtttcaa
     4561 ccaattggcg tttgataaaa ccagctttat caagttcact caaacctcca cgttcagctt
     4621 tcgttaaatt atcaaactta cgttgagtga ttaacttggc gtttagaagt tgtctccaat
     4681 agtttttcat ctttttgact acttcttcac ttggaacgtt atccgattta ccacgatttt
     4741 tatcagaacg cgttaagacc ttattgtcta ttgaatcgtc tttaaggaaa ctttgtggaa
     4801 caatggcatc gacatcataa tcacttaaac gattaatatc taattcttgg tccacataca
     4861 tgtctcttcc attttggaga taatagagat agagcttttc attttgcaat tgagtatttt
     4921 caacaggatg ctctttaaga atctgacttc ctaattcttt gataccttct tcgattcgtt
     4981 tcatacgctc tcgcgaattt ttctggccct tttgagttgt ctgattttca cgtgccattt
     5041 caataacgat attttctggc ttatgccgcc ccattacttt gaccaattca tcaacaactt
     5101 ttacagtctg taaaatacct tttttaatag cagggctacc agctaaattt gcaatatgtt
     5161 catgtaaact atcgccttgt ccagacactt gtgctttttg aatgtcttct ttaaatgtca
     5221 aactatcatc atggatcagc tgcataaaat tgcgattggc aaaaccatct gatttcaaaa
     5281 aatctaatat tgttttgcca gattgcttat ccctaatacc attaatcaat tttcgagaca
     5341 aacgtcccca accagtataa cggcgacgtt taagctgttt catcacctta tcatcaaaga
     5401 ggtgagcata tgttttaagt ctttcctcaa tcatctccct atcttcaaat aaggtcaatg
     5461 ttaaaacaat atcctctaag atatcttcat tttcttcatt atccaaaaaa tctttatctt
     5521 taataatttt tagcaaatca tggtaggtac ctaatgaagc attaaatcta tcttcaactc
     5581 ctgaaatttc aacactatca aaacattcta tttttttgaa ataatcttct tttaattgct
     5641 taacggttac ttttcgattt gttttgaaga gtaaatcaac aatggctttc ttctgttcac
     5701 ctgaaagaaa tgctggtttt cgcattcctt cagtaacata tttgaccttt gtcaattcgt
     5761 tataaaccgt aaaatactca taaagcaaac tatgttttgg tagtactttt tcatttggaa
     5821 gatttttatc aaagtttgtc atgcgttcaa taaatgattg agctgaagca cctttatcga
     5881 caacttcttc aaaattccat ggggtaattg tttcttcaga cttccgagtc atccatgcaa
     5941 aacgactatt gccacgcgcc aatggaccaa cataataagg aattcgaaaa gtcaagattt
     6001 tttcaatctt ctcacgattg tcttttaaaa atggataaaa gtcttcttgt cttctcaaaa
     6061 tagcatgcag ctcacccaag tgaatttgat ggggaataga gccgttgtca aaggtccgtt
     6121 gcttgcgcag caaatcttca cgatttagtt tcaccaataa ttcctcagta ccatccattt
     6181 tttctaaaat tggtttgata aatttataaa attcttcttg gctagctccc ccatcaatat
     6241 aacctgcata tccgtttttt gattgatcaa aaaagatttc tttatacttt tctggaagtt
     6301 gttgtcgaac taaagctttt aaaagagtca agtcttgatg atgttcatcg tagcgtttaa
     6361 tcattgaagc tgatagggga gccttagtta tttcagtatt tactcttagg atatctgaaa
     6421 gtaaaatagc atctgataaa ttcttagctg ccaaaaacaa atcagcatat tgatctccaa
     6481 tttgcgccaa taaattatct aaatcatcat cgtaagtatc ttttgaaagc tgtaatttag
     6541 catcttctgc caaatcaaaa tttgatttaa aattaggggt caaacccaat gacaaagcaa
     6601 tgagattccc aaataagcca tttttcttct caccggggag ctgagcaatg agattttcta
     6661 atcgtcttga tttactcaat cgtgcagaaa gaatcgcttt agcatctact ccacttgcgt
     6721 taatagggtt ttcttcaaat aattgattgt aggtttgtac caactggata aatagtttgt
     6781 ccacatcact attatcagga tttaaatctc cctcaatcaa aaaatgacca cgaaacttaa
     6841 tcatatgcgc taaggccaaa tagattaagc gcaaatccgc tttatcagta gaatctacca
     6901 atttttttcg cagatgatag atagttggat atttctcatg ataagcaact tcatctacta
     6961 tatttccaaa aataggatga cgttcatgct tcttgtcttc ttccaccaaa aaagactctt
     7021 caagtcgatg aaagaaacta tcatctactt tcgccatctc atttgaaaaa atctcctgta
     7081 gataacaaat acgattcttc cgacgtgtat accttctacg agctgtccgt ttgagacgag
     7141 tcgcttccgc tgtctctcca ctgtcaaata aaagagcccc tataagattt tttttgatac
     7201 tgtggcggtc tgtatttccc agaaccttga actttttaga cggaacctta tattcatcag
     7261 tgatcaccgc ccatccgacg ctatttgtgc cgatagctaa gcctattgag tatttcttat
     7321 ccatttttgc ctcctaaaat gggccctgca accattatca ccgccagagg taaaatagtc
     7381 aacacgcgcg gtgttagata tttatccctt gcggtgatag atttaacgtt aaggaggtat
     7441 gtatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca cgtcgcctta
     7501 aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca
     7561 agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat gcattaaatg
     7621 cttataacgc cgcattgctt acaaaaattc tcaaagttag cgttgaagaa tttagccctt
     7681 caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg tcacttagaa
     7741 gtgagtatga gtaccctgtt ttttctcatg ttcaggcaga gatgttctca cctaagctta
     7801 gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa gccagtgatt
     7861 ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc tccaagccaa
     7921 gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag ccaggtgatt
     7981 tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc agggatagcg
     8041 gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc aatgagagtt
     8101 gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt ggctgatcgg
     8161 caaggtgttc tggtcggcgc atagctgata acaattgagc aagaatcttc atcgctagac
     8221 cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatccac
     8281 gcgtttacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     8341 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     8401 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     8461 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     8521 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     8581 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     8641 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     8701 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa
     8761 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     8821 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     8881 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     8941 ccattttagc ttccttagct cctgaaaatc tcgacggatc ctaactcaaa atccacacat
     9001 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc cctctagcta
     9061 ccggttttgg tcatgagatt atctctgtgc atatggacag ttttcccttt gatatgtaac
     9121 ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag
     9181 ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt
     9241 ttttgcgtga gccatgagaa cgaaccattg agatcatact tactttgcat gtcactcaaa
     9301 aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt
     9361 cttagtccgt tatgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc
     9421 atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact
     9481 tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg
     9541 taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca
     9601 tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt
     9661 gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag
     9721 tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg
     9781 aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg
     9841 gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca
     9901 gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg
     9961 atgttcatca tctgaacgta ttggttataa gtgaacgata ccgtccgttc tttcattgta
    10021 gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc
    10081 tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac
    10141 atacatctca attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa
    10201 tgataattac tagtcctttt cctttgagtt gtgggtatct gtaaattctg ctagaccttt
    10261 gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt
    10321 ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata
    10381 aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc gacgtgcaca
    10441 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    10501 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
    10561 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    10621 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
    10681 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
    10741 tgcgtaagga gaaaataccg catcaggcga ttatgtcttt tgcgcattca cttcttttct
    10801 atataaatat gagcgaagcg aataagcgtc ggaaaagcag caaaaagttt cctttttgct
    10861 gttggagcat gggggttcag ggggtgcagt atctgacgtc aatgccgagc gaaagcgagc
    10921 cgaagggtag catttacgtt agataacccc ctgatatgct c
//
