LOCUS       sequence-220948-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes HO 3' Homology as a Type 7 part to be used in the Dueber YTK
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK088
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(868..887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcat cggggcagaa ctaactcttt atttttccaa
      181 atcagaaaaa ttaatatgtt ttgccgcgtt aaaacctaca tcaaaaaagg cggatcaaga
      241 tgtatgaaag aaagtgcgta gaataacgaa cattcatagc tgttctggag gctttacaaa
      301 aggtaatctt tgttaggtgc gattttatcc gaaaagcaat tactctctat gttagtcata
      361 caaactgact tctgtgagag aattattttc atatcaacgt aagatcacat ggttccttta
      421 tcaagtacta ctatcattcc attatatgac ctatttactt cttgaatctt agagctcata
      481 attcaagcaa gttgcggagc taagaatttc acatgttgtt gaacttaaca atcttcatta
      541 tacccaatcg ctgcgtgctg gaattatgtt aaaagttaca tccttttttt catttttccc
      601 tacgctcagg gcactgtact gcccgtgcct gcgatgagat acatcaattt aaagccgcag
      661 atgcaacacg ataactctga gaccgactac ggttatccac agaatcaggg gataacgcag
      721 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      781 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      841 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      901 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      961 cgggaa
//
