LOCUS       sequence-220951-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes HO 5' Homology as a Type 8b part to be used in the Dueber 
            YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK094
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      102..136
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(868..887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt ctggtctcaa gggacagcga tggaacttac ggcaaagacc
      181 tcaacagtaa ttaacccaaa ggggtatcaa ataatcgatg tgctttttca ctctacgaat
      241 gatctgtgag aaactgattt gggccgaatc gcgtaaaaag tttgattcgt ggcggctaat
      301 gtctgagggg ctccaacagg ctcgtagagc ctcgtttctt gagggcacaa aatgtccagg
      361 taatattccc aagaaagaac cgcagagtgc tttgataaat cggttacagg tcttaacgta
      421 ggttttgtct cgctaattgc tattgagtaa gttcgatccg tttggcgtct tttggggtgt
      481 aacgccaaac ttattacttt tcctatttga ggttggtatt gattgttgtc aaagaatgaa
      541 aatatacaca aacgccacaa tatacgtacc aggttcacga aaactgatcg tatggttcat
      601 accctgactt ggcaaaccta atgtgaccgt cgctgattag cggatcacga aaagtgatct
      661 cgatacaatt agaattgtga gaccgactac ggttatccac agaatcaggg gataacgcag
      721 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      781 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      841 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      901 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      961 cgggaa
//
