LOCUS       sequence-220953-        4247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: AAEL009024 PDF R
FEATURES             Location/Qualifiers
     source          1..4247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2135..2269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2172..2191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2226..2245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2308..2325)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2479..3067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2559..2578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3238..4029,4030..4098))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3861..3880
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4099..4203)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4243..4247,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat ttcctcgaga tggttcctta ctgcaactgg acatgggacg acgtcctttg
      841 ttggcctcct acccccgcgg gaaaggcaat tcggcaaccg tgtccctctg ggcatggtat
      901 agataccagc aaatttgcag aaagaagatg tagcgctgaa ggccggtggg agggcagacc
      961 aggaactaca ccaactccac ttggatggac caactacaca ccatgctata gccccgaggt
     1021 cattcaatta tttaggaaac tttattccgg caccggtggc aatgacgaag ctgatatcaa
     1081 aatagatatt gccgaacgaa cgcgaacact ggaaattgtg gggtttagtc tttcattgat
     1141 agcacttatt atatcgttgg tgatattctg tagatttaga agtctacgca acaacagaac
     1201 gcgcattcac aaaaacctct tcgtggccat ggtaatacag gtgataatta gattaacgtt
     1261 gtacatcgac caagcaatta tccgaagcgg aagcaaagga tacggcaacg aaagctcccg
     1321 tcatggcata gacaacacgc cttacctttg cgaggcctca tatatattgt tggagtacgc
     1381 caggacctgc atgttcatgt ggatgttcat agaaggatta tatttacaca acgtggtcac
     1441 cgtgacagtg ttccagggcc gctttccaca cacactgtac gcgatggtcg gatggggagg
     1501 tcctgtggca ctgaccgcca tttgggctgt taccaccgca caatacgtag gccagcagaa
     1561 atgctggtgg ggatacaacc taacgcctta ttactggatt ctagaaggtc caagattagc
     1621 tgtagttctg ttaaacttca tatttttact gaatattata agagtgttaa tcgttaagct
     1681 gcgtcagagt cacacaagcg atgtggagca agtgcgaaaa gcggtgcgtg cggctgttgt
     1741 tctagtgcca ttattaggga taacaaattt actcaacatg actgaggcac cgttggatag
     1801 aacagcgctg gagtttgccc tttggtcgta cgtgacgcac ttcctaacat cgttccaggg
     1861 attttttatt gctctgctgt attgcttcct aaatggagag gtccgaatag cgttaatgaa
     1921 aagtctcagc gtatatctct caattagggg acaccatgat tggcccgtcc gaaggccttc
     1981 aatgttttct gctgcatacg gcacagcacc agaaaccgaa ggacccaatg cagtcgattt
     2041 gaaacggtga gcggccgcta gactagtcta gagaaaaaac ctcccacacc tccccctgaa
     2101 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     2161 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     2221 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc ccgggtaccg
     2281 agctcgaatt aattcctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2341 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2401 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2461 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2521 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2581 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2641 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttg
     2701 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2761 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2821 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2881 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2941 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3001 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3061 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3121 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3181 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3241 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3301 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3361 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3421 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3481 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3541 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3601 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3661 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3721 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3781 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3841 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3901 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3961 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4021 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4081 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4141 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4201 gcgcacattt ccccgaaaag tgccacctgc aagcttggct gtggaat
//
