LOCUS       sequence-220966-        5593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: AAEL004777  Bursicon R
FEATURES             Location/Qualifiers
     source          1..5593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    3481..3615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3518..3537)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3572..3591
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3654..3671)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3825..4413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3905..3924)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4584..5375,5376..5444))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5207..5226
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5445..5549)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(5589..5593,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attccggaat tcctcgagat gacaacccac gccaatttcc gttttctctc tgggggccat
      841 tgggaaaacg acagcgacat ggaagcgaac catatctact tcatccacca ggacgccttc
      901 aggccgctaa tcaaactgga agatttgtat gtgtcttgct gtttgagatt cactatagca
      961 aacactttct attgacaagg ataccacaaa tcacttgact ggttttttcc ttcttttctc
     1021 tttttcagaa acctgggaaa taatttgttt cccaatcttc ccacggcagg gttggcacgt
     1081 ctgctacacc tgaaaacgtt caacaaccca aatctgaggg agtttccgcc accagagtcc
     1141 tttccccgca ttcagacact tgtgctctcc tacgcttacc actgctgctc gtttctgccg
     1201 cttgtggcca gtgctccaaa agttcctaag catcatccta tcaaggagga ggtccttttc
     1261 ccgactgaca acgagttcga tatgagcttg tggaacaata gctacaatga catctggcca
     1321 cagttacata atttaagcaa aaagtttgga acgcagatta atgaactgct gcacgcttat
     1381 ggtcctgagt actatccagg aggaatgcca acctatccgg aagagtactt tgaagaggaa
     1441 ggcggcgtca gtcatcccat tcccacatca caaccgggaa cgattcaatg tgtaccagag
     1501 ccgggacctt tcatgccctg tcaagacctt ttcgactggt ggacccttcg ttgtggcgtt
     1561 tgggttgtgt tcctgctggc gatgctcggg aatgggacgg tggtgtttgt gctcatcttc
     1621 tccagatcaa aaatggacgt gccacggttt ctggtgtgca acttggctgc tgcggatttc
     1681 ttcatgggaa tctacttggg atttttggca gtagtagatg cttcaacact cggggagttt
     1741 cgcatgtacg caattccttg gcagatgagc gccgggtgca agctgtctgg atttctggca
     1801 gtactttcat cggaactatc agtttacacc ttggctgtga ttactctgga aagaaactac
     1861 gctatcaccc acgccatgca tttaaacaag agactatcgt taaggcatgc cgggtatatc
     1921 atgacaatag gatggacctt tgctataaca atggcaatac taccgttgtt cggagtttca
     1981 gattaccgtg tattcgctgt atgtcttcca tttgaagtta caagtggaac cggtagctta
     2041 gcctacgttg tatttctgat gttcatcaac ggagtagcgt ttctcatatt aatgggatgc
     2101 tacttaaaaa tgtactgcgc aatccgaggt tcccaagcgt ggaattccaa cgattcccga
     2161 atagcaaagc gaatggcatt gctagtcttc acggacttca tctgttggtc cccgatagcg
     2221 ttcttctcgc tgacagccgt ttttgggctg catctcatct ctctagaaca agccaaagtc
     2281 ttcacagtct tcattctacc tctcaactcc tgctgcaacc cgttccttta tgccattcta
     2341 actaaacagt tcaagaaaga ctgcgtcctc atctgcaagg caattgagga atcacgtgta
     2401 acgcgtggca ttggccgctg tcgccacagt tctaacttca gcaaccgtca tacacctgcc
     2461 aacacaaatt cactggtaga gagatcctct cgtgaactgc cgccacttct accaggtcaa
     2521 acatgtaact gttcagcgaa gctaatggaa caggagaatc tccgtcgacg tctccaacag
     2581 cagagtacat tttccaaaac gtggcgcagg ttactgtgca acctagggca acgtaacgat
     2641 ggccgtcgtg gaagacggaa cggtgatcaa tatgcgtacc aaatcgcaga aatccaacag
     2701 aaacagcaca aacgggccgg atcggtatcg tccagtgaaa acttcagttc atctcggtcc
     2761 gactcgtggc gacacgccca gcatcattgt ggaattcctc tgcggttgct ggatccacgg
     2821 cggaggcaca cttcgtggtt gatcacaaga aaaacttccc aggattcgaa tctgtccagc
     2881 tcgaggaatg attcgtccgg atcaaccgct acgcagagta cgggaacgtg gcgaatatca
     2941 cggtccagtg ccagcaattc ggtagttctg cccggggtga aaatcgatgg cgtaccccat
     3001 ccacatcata caagtggcgc acgccaatca atcccaggtg gaaagccccg tctcgtgcgg
     3061 cagtcagccg ttcaggatga ttcacaggag ctgtcctgtt caccgccccg attgggtgta
     3121 cgtttcttgc ccacgattcc gtcggcagcc gatagcagta ttcagctgga cgacgacacg
     3181 gccgaagccc gctccagtcc ctcgtcgtgt cacagtgaac ccgccatttg cgatgtggat
     3241 gtggaagcgg caggaaccgg aaccagtgcc gaaggtgccc agcaacagca atcgcagcag
     3301 caaaacatgt ctcccgcccc cttctatgcc atccttcacg ggagcaatcc tcaggcaacc
     3361 gtttctagtc tcaaagataa aaccaagccg ccgtgagcgg ccgctagact agtctagaga
     3421 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     3481 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3541 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3601 tatcatgtct ggatccccgg gtaccgagct cgaattaatt cctcttccgc ttcctcgctc
     3661 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3721 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3781 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3841 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3901 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3961 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4021 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4081 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4141 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4201 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4261 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4321 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4381 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4441 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4501 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4561 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4621 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4681 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4741 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4801 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4861 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4921 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4981 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5041 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5101 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5161 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5221 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5281 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5341 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5401 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5461 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5521 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcaagc
     5581 ttggctgtgg aat
//
