LOCUS       sequence-220969-        4598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Ae. aegypti receptor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Duvall LB, Ramos-Espiritu L, Barsoum KE, Glickman JF, Vosshall LB
  TITLE     Small-Molecule Agonists of Ae. aegypti Neuropeptide Y Receptor Block
            Mosquito Biting.
  JOURNAL   Cell. 2019 Feb 7;176(4):687-701.e5. doi: 10.1016/j.cell.2018.12.004.
  PUBMED    30735632
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.12"; journalName: "Cell"; date: "2019-02-7- 7";
            volume: "176"; issue: "4"; pages: "687-701.e5"
COMMENT     Sequence Label: AAEL008292 DH44R
FEATURES             Location/Qualifiers
     source          1..4598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..330,331..345,346..614)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             346..614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          644..740
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2486..2620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2523..2542)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2577..2596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2659..2676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2830..3418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2910..2929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3589..4380,4381..4449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4212..4231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4450..4554)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(4594..4598,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg atcgaggggc tcgcatctct
      361 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      421 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      481 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      541 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      601 cgccgttaca gatccaagct ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt
      661 gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg ctcctcagtg
      721 gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagctgcgga
      781 attcctcggc aagtgataac gataagaagt gctccgatta gtgtaggacc caagtgtcct
      841 caacaaggat gaacgactca tcatcagcgt cagtggagtc gttgttagcc ctggcgggtg
      901 ccggtggaga tttcgccggt tccggtagtg gcgatggctt tccaccaatg attttgaacg
      961 aaactttgcg ggatctgtgc caccaacaga acgacaccct gtttctggag ctgtcctgtc
     1021 ctccattgtt cgattcgatc tcgtgctggc caaggacacc gccggcaaca acggcagttt
     1081 tgccgtgttt ctccgagttc aagggagtgc agtacgatgc gacacagaac gccacccgct
     1141 actgcaacat cgacggaacg tggaacagct tcgcgaacta cgatgcctgc aagcacctgg
     1201 aactgccatc gtccgatccg acgctcgaat cgttcatcga attaccgata gtgatttatt
     1261 ttgtagggta taccataagc ttactggcgc ttttttgcgc cgttaccgtt ctagtttact
     1321 ttaaggagct acgatgcctc aggaatacta ttcacgtcaa tctcttcgtc acctacatca
     1381 tgtcgtccag cctgtggatc atcatactct ctcaacaggt gtttagttgg agcaattatg
     1441 tttgtaaacg ttttgtctcc gataaccgat ttatgccgtt acgcttcttg ttttttctct
     1501 tccgtttttt ttcttctttt tatcgctttt ctacttgcag ttggctgcca agcagggtct
     1561 ggtggattgc atcttcctgg tgacgctatt ccactacttt agtacgacca acttcttctg
     1621 gatgctggtg gaaggtctct atctctacat gctggtggtt caaacgttct ccggcgatta
     1681 tctacgcttt tggaagtatt ccatcatcgg ctggggaggt cctttaatct ttgtcggagc
     1741 gtgggccatt gccaagtcct tttatccgta tgacctgatg ccggagcatc cgaacaagct
     1801 ggaaatcgaa tgctcgtgga tgcgggaatc tcacatcgac tggattattc agggcccatc
     1861 ctgtgccgtg ctagtaatca atctcatctt cctgctgcga atcatgtggg ttctcatcac
     1921 caagctccga tcggccaata cggtcgaaac gaggcaatac cggaaggcat ccaaggcact
     1981 gctggtactt attccgctgc taggcatcac ctatctggtg gtcatctacg ggcctcacga
     2041 gggcgttggc agtcggatct ttgcggtcac cagagcggtc ctgttgagca cacagggctt
     2101 ggtagtatcg ttactctact gcttcctcaa ctatgaggtg cgaggaactc tacggttaca
     2161 ctactaccgt tggcgagatg agcgcaacat tcgactgggc atcatttcca aacatcgaag
     2221 accgaccatc agtggaactg aaacaccctg gagtatagtg tcgtgataat gaaaagcttg
     2281 gcaccaccct cctcaaggca ccaccaccac cacaaccaca accatcacaa taaccttggc
     2341 ggcagcaact gaaattcagc ttccagtcaa atttgtacta tggaccttcg gacaagtttg
     2401 ggcggccgct agactagtct agagaaaaaa cctcccacac ctccccctga acctgaaaca
     2461 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     2521 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     2581 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cccgggtacc gagctcgaat
     2641 taattcctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4561 tccccgaaaa gtgccacctg caagcttggc tgtggaat
//
